Multiplex Multiplex Assay



Human Acute Kidney Injury Antibody Array - Membrane (20 targets) - ab169806 from Abcam

Category Antibody Array
Prices $500.00, $1,112.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Human Angiogenesis Antibody Array - Membrane (20 Targets) - ab134000 from Abcam

Category Antibody Array
Prices $395.00, $672.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Human Cytokine Antibody Array - Membrane. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Human Angiogenesis Antibody Array - Membrane (23 targets) - ab169808 from Abcam

Category Antibody Array
Prices $395.00, $680.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Membrane Antibody Arrays. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Human Apoptosis Antibody Array - Membrane (43 Targets) - ab134001 from Abcam

Category Antibody Array
Prices $335.00, $500.00, $950.00
Sizes 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes
Apoptotic protein profiling in HeLa cell lines.400 μg of cell lysates from Human MD231, BT549 and HeLa cell lines were incubated overnight with ab134001 Human Apoptosis Antibody Array - Membrane. Control membrane was incubated with blocking buffer. The antibody array membranes were then washed and cocktail of biotinylated antibody mix was used to detect apoptosis-related proteins. After incubation with HRP-Conjugated Streptavidin, the signals were visualized after 5 minute exposure by chemiluminescene.

Human Atherosclerosis Antibody Array - Membrane (20 targets) - ab169803 from Abcam

Category Antibody Array
Prices $500.00, $770.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Human Chemokine Antibody Array - Membrane (38 targets) - ab169812 from Abcam

Category Antibody Array
Prices $500.00, $950.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Human Cytokine Antibody Array - Membrane (23 Targets) - ab133996 from Abcam

Category Antibody Array
Prices $500.00, $680.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133996. Media alone was used as a negative control.

Human Cytokine Antibody Array - Membrane (36 targets) - ab169811 from Abcam

Category Antibody Array
Prices $500.00, $950.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines.

Human Cytokine Antibody Array - Membrane (42 Targets) - ab133997 from Abcam

Category Antibody Array
Prices $500.00, $1,045.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control.

Human Cytokine Antibody Array - Membrane (49 targets) - ab169814 from Abcam

Category Antibody Array
Prices $590.00, $830.00, $1,411.00
Sizes 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes
Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Human Cytokine Antibody Array - Membrane (51 targets) - ab169815 from Abcam

Category Antibody Array
Prices $590.00, $830.00, $1,411.00
Sizes 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes
Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Human Cytokine Antibody Array - Membrane (54 targets) - ab169816 from Abcam

Category Antibody Array
Prices $500.00, $750.00, $1,425.00
Sizes 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes
Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Human Cytokine Antibody Array - Membrane (60 targets) - ab169817 from Abcam

Category Antibody Array
Prices $500.00, $750.00, $1,425.00
Sizes 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes
Typical images obtained with Abcam Human Cytokine Antibody Array. The membranes were probed with conditioned media from two different cell lines and a mock buffer control. Membranes were exposed to film at room temperature for 1 minute.

Human Cytokine Antibody Array - Membrane (60 targets) - ab169818 from Abcam

Category Antibody Array
Prices $500.00, $750.00, $1,425.00
Sizes 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes
Typical images obtained with Abcam Human Cytokine Antibody Array. The membranes were probed with conditioned media from two different cell lines or a mock buffer control. Membranes were exposed to film at room temperature for 1 minute.

Human Cytokine Antibody Array - Membrane (80 Targets) - ab133998 from Abcam

Category Antibody Array
Prices $500.00, $750.00, $1,425.00
Sizes 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133998.

Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) - ab134005 from Abcam

Category Antibody Array
Prices $275.00, $500.00, $720.00
Sizes 1 x 2 membranes, 1 x 4 membranes, 1 x 8 membranes
Human epidermoid carcinoma cell line, A431 cells that were 80-90% confluent were serum starved overnight, then exposed to 100 ng/ml EGF for 20 minutes at 37°C. Control cells were serum starved without the subsequent stimulation with EGF. Cell lysates were prepared according to the protocol. To use ab134005 Human EGFR Phosphorylation Antibody Array - Membrane, treated or untreated cell lysate was added into antibody array membrane. The antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals were visualized by chemiluminescence.

Human Growth Factor Antibody Array - Membrane (41 Targets) - ab134002 from Abcam

Category Antibody Array
Prices $550.00, $990.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Human Inflammation Antibody Array - Membrane (20 targets) - ab169804 from Abcam

Category Antibody Array
Prices $500.00, $770.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control.

Human Inflammation Antibody Array - Membrane (20 targets) - ab169805 from Abcam

Category Antibody Array
Prices $500.00, $750.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Membrane Antibody Arrays. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Human Inflammation Antibody Array - Membrane (40 Targets) - ab134003 from Abcam

Category Antibody Array
Prices $500.00, $950.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA.Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control.

Human MMP Antibody Array - Membrane (10 Targets) - ab134004 from Abcam

Category Antibody Array
Prices $395.00, $648.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Membrane Antibody Arrays. Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min. Positive control spots are pinted with biotinylated antibody to normalize and orientate the araays. Negative controls spots are printed with buffer to measure the baseline response (used to determine the level of non-specific binding of the samples). Blank spots printed with nothing to measure the background response.

Human Obesity Antibody Array - Membrane (62 targets) - ab169819 from Abcam

Category Antibody Array
Prices $750.00, $1,100.00
Sizes 1 x 4 membranes, 1 x 8 membranes
5 mg of lyophilized microdissected skeletal muscle (vastus laterals) was homogenized in 180 uL 1X cell lysis buffer (included in kit). After a 10,000 x g 5 min spin at 4°C removed the supernatant was removed and the total protein determined. 250 µg of total protein was added to each membrane with overnight incubations for each incubation step. The attached image is the first blot setting up the method and was exposed using a FluorChem M for 27 seconds. As can be seen Acrp30 will require less protein/less exposure but otherwise all but seven cytokines were visualized following this protocol.Abreview rating 5/5 stars. Review from Abcam user community. Verified customer at Baltimore VA Medical Center. Submitted Oct 9 2014.

Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) - ab169809 from Abcam

Category Antibody Array
Prices $500.00, $850.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Mouse Angiogenesis Antibody Array - Membrane (24 Targets) - ab139697 from Abcam

Category Antibody Array
Prices $500.00, $880.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Mouse Atherosclerosis Antibody Array - Membrane (22 targets) - ab169807 from Abcam

Category Antibody Array
Prices $500.00, $820.00
Sizes 1 x 4 membranes, 1 x 8 membranes
Typical images obtained with Abcam Mouse Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.
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