Apoptotic protein profiling in HeLa cell lines.400 μg of cell lysates from Human MD231, BT549 and HeLa cell lines were incubated overnight with ab134001 Human Apoptosis Antibody Array - Membrane. Control membrane was incubated with blocking buffer. The antibody array membranes were then washed and cocktail of biotinylated antibody mix was used to detect apoptosis-related proteins. After incubation with HRP-Conjugated Streptavidin, the signals were visualized after 5 minute exposure by chemiluminescene.
Apoptotic protein profiling in Induced and uninduced Jurkat cell lines.Jurkat cells were treated with apoptosis inducer set for 7 hours (10mM Actinomycin D, 2mM Camptothecin, 100mM Cycloheximide, 10mM Dexamethasone, and 100mM Etopiside). 500 µg of cell lysate from both induced and uninduced Jurkat cells were incubated overnight with ab134001 Human Apoptosis Antibody Array - Membrane. Control membrane was incubated with blocking buffer. The antibody array membranes were then washed and cocktail of biotinylated antibody mix was used to detect apoptosis-related proteins. After incubation with HRP-Conjugated Streptavidin, the signals were visualized after 5 minute exposure by chemiluminescence.