Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA.Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control.
Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.
Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003.
Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003. Mean pixel density was quantified using CCD camera software analysis.
Typical results obtained with Abcam Membrane Antibody Arrays: Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.