IHC Immunohistochemistry



CD45 (136-4B5) Mouse mAb - 3575 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 136-4B5
Immunohistochemical analysis of paraffin-embedded human tonsil using CD45 (136-4B5) Mouse mAb.

Anti-200 kD Neurofilament Heavy antibody - ab4680 from Abcam

Prices $401.00
Sizes 25 µl
Host Chicken
Clonality Polyclonal
Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section). 

Anti-200 kD Neurofilament Heavy antibody [NF-01] - ab7795 from Abcam

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone NF-01
ab7795 at a dilution of 1/1000, staining 200 kD Neurofilament Heavy  (Alexa 488 secondary at 1/2000) in rat brain tissue (30µm thick coronal sections) on free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

Anti-200kDa + 160kDa Neurofilament (phospho ) antibody [SMI 34] - ab24571 from Abcam

Host Mouse
Clonality Monoclonal
Clone SMI 34
ab24571 staining 200kDa + 160kDa Neurofilament (phospho) in mouse brain tissue sections by Immunohistochemistry (resin sections). Samples were incubated with primary antibody (1/500 in PBS + £% normal goat serum) for 20 hours at 4°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.See Abreview

Anti-ABCD4 antibody - ab81905 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-ABCD4 antibody (ab81905) at 0.3 µg/ml + Jurkat Lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-Actin antibody [5J11] - ab190301 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5J11
immunofluorescent analysis of HeLa cells labeling Actin with ab190301 at 1/1000 dilution (green) and chicken polyclonal antibody to Vimentin (red). The blue stain reveals DNA in the nuclei of these cells.

Anti-active + pro Caspase 3 antibody - ab13847 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab13847 staining caspase 3 in SKNSH cells treated with Z-IETD-FMK (ab141382), by ICC/IF. Decrease in caspase 3 expression correlates with increased concentration of Z-IETD-FMK, as described in literature.The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141382 (Z-IETD-FMK) in DMSO. After this incubation, 10 μM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24 hours. The samples were then fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A goat anti-rabbit DyLight 488 (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-ADAM33 antibody - Cytoplasmic domain - ab39193 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab39193 staining human normal myometrium. Staining is localized to the membrane.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-ADP Ribosylation Factor antibody [1D9] - ab2806 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1D9
Immunocytochemistry/Immunofluorescence analysis of ADP Ribosylation Factor shows staining in HeLa cells. ADP Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2806 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Anti-AGBL1 antibody - ab106237 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal stomach tissue labelling AGBL1 with ab106237 at a dilution of 1/100.

Anti-AKT1/2 antibody - ab6076 from Abcam

Prices $370.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab6076 detecting strong nuclear staining of AKT1/2 in adult dorsal root ganglion cells cultured for 2 days. ab6076 was used at 1/300 (incubated for 2h at room temperature). Secondary antibody used is anti-rabbit alexa fluor 488 (at 1/1000 for 1hr at room temperature). The cell diameter is approx. 25µm. Nucl = Nucleus; Cyt.=Cytoplasm.

Anti-ALDH1L1 antibody - ab190298 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of human neuron-glial cell mixture cultures labeling ALDH1L1 with ab190298 at 1/2000 (red) and a monoclonal antibody against GFAP (green). Blue is a DNA stain. Astrocytes labeled by ab190298 and anti-GFAP appear yellow. 

Anti-ALDH3B2 antibody - ab112527 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal liver tissue labelling ALDH3B2 with ab112527 at a dilution of 1/100.

Anti-Aldolase C antibody [4A9] - N-terminal - ab190368 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A9
Immunofluorescence analysis of neuron/glial cells, labeling Aldolase C using ab190368 at a 1/500 dilution (green). Counterstained with an antibody against NeuN (red) and DNA (blue).

Anti-alpha 2a Adrenergic Receptor antibody - ab65833 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC - Wholemount of Zebrafish embryo hair cell labelling alpha 2a Adrenergic Receptor with ab65833. Sample was incubated with primary antibody (1/1000) for 18 hours at 4°C. An undiluted Alexa Flour® 568-conjugated Goat anti-rabbit IgG monoclonal was used as the secondary antibody.See Abreview

Anti-alpha Actinin antibody [0.T.02] - ab18061 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 0.T.02
Anti-alpha Actinin antibody [0.T.02] (ab18061) at 1/2000 dilution + Human pancreatic cancer cell line - whole cell lysate at 20 µgSecondaryHRP conjugated goat anti-mouse antibody at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker - ab52866 from Abcam

Prices $404.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1332Y
alpha Tubulin was immunoprecipitated from 1mg of HeLa whole cell extract using ab52866 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab52866 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1 Hela whole cell extract, Lane 2 PBS instead of whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

Anti-Angiopoietin like 7 antibody - ab118198 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal pancreas tissue labelling Angiopoietin like 7 with ab118198 at a dilution of 1/100.

Anti-Annexin A3 antibody - ab33068 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Annexin A3 antibody (ab33068) at 1.25 µg/ml + placenta lysate at 10 µgSecondaryHRP conjugated anti Rabbit IgG at 1/50000 dilution

Anti-Anterior Gradient 2 antibody - ab181339 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling Anterior Gradient 2 with ab181339 at 5 µg/ml.

Anti-AP2 gamma antibody [3B5] - ab110635 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3B5
Anti-AP2 gamma antibody [3B5] (ab110635) at 1/100 dilution + MCF7 cell lysate

Anti-Artemin antibody - ab101952 from Abcam

Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of cleavage-stage Human embryo, staining Artemin (green) with ab101952. Embyros were fixed with 4% paraformaldehyde for 30 min at 23°C. After permeabilization with 0.1% Triton X-100, embryos were pre-incubated in 5% BSA for 1 hour before incubation with primary antibody (diluted in PBS with 1% BSA) overnight at 4°C. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody.

Anti-ATM antibody [5C2] - ab2618 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5C2
Anti-ATM antibody [5C2] (ab2618) at 1/5000 dilution + lysate prepared from HEK293 cells over-expressing human FLAG tagged ATM at 0.05 µgSecondaryHRP conjugated goat anti-mouse polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Aurora A antibody [1A11] - ab190367 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A11
Immunofluorescence analysis of HeLa cells, labeling Aurora A using ab190367 at a 1/100 dilution (green). Counterstained with an antibody against Vimentin (red) and DNA (blue).

Anti-beta Catenin antibody - ab6302 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC-Wholemount image of anti-beta Catenin antibody (ab6302) staining on the Zebrafish jaw of a 5 day old larva. This larva was stained with anti-beta Catenin antibody (green) and a collagen 2 antibody (red) for 2 hours at 20°C. The image shows the cartilage elements of the jaw.See Abreview
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