VPRBP

Gene Symbol VPRBP
Entrez Gene 9730
Alt Symbol DCAF1
Species Human
Gene Type protein-coding
Description Vpr (HIV-1) binding protein
Other Description DDB1 and CUL4 associated factor 1|DDB1- and CUL4-associated factor 1|HIV-1 Vpr-binding protein|Vpr-binding protein|protein VPRBP|serine/threonine-protein kinase VPRBP|vpr-interacting protein
Swissprots Q9HCA1 Q8TBD9 Q2YD74 Q9Y4B6 Q9UG37
Accessions EAW65140 EAW65141 EAW65142 EAW65143 Q9Y4B6 AB018343 BAA34520 AF061935 AAG27134 AF086402 AK023239 BAG51173 AL080145 CAB45738 BC022792 AAH22792 BC110371 AAI10372 BP222559 CR986856 XM_005276753 XP_005276810 XM_005276755 XP_005276812 XM_006713428 XP_006713491 XM_011534273 XP_011532575 XM_011534274 XP_011532576 XM_011534275 XP_011532577 XM_011534276 XP_011532578 XM_011534277 XP_011532579 XM_011534278 XP_011532580 XM_011534279 XP_011532581 XM_011534280 XP_011532582 XR_940521 XR_940522 XR_940523 XR_940524 XR_940525 NM_001171904 NP_001165375 NM_014703 NP_055518
Function Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1- CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1- DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: VPRBP is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT
Subcellular Location Cytoplasm. Nucleus. Note=Associated with chromatin in a DDB1-independent and cell cycle-dependent manner: recruited to chromatin as DNA is being replicated and is released from chromatin before mitosis.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:11223251}.

VPRBP Antibody - 13931 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using VPRBP Antibody.

VPRBP (D5K5V) Rabbit mAb - 14966 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5K5V
Western blot analysis of extracts from various cell lines using VPRBP (D5K5V) Rabbit mAb.

VPRBP (C-8) - sc-376850 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPRBP (C-8): sc-376850. Western blot analysis of VPRBP expression in BJAB whole cell lysate.

VPRBP (K-20) - sc-82419 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

VPRBP (H-300) - sc-98531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VPRBP (H-300): sc-98531. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic and membrane staining of neuronal cells.

Anti-VPRBP antibody - ab75458 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VPRBP antibody (ab75458) at 1 µg/mlLane 1 : Liver (Rat) Tissue Lysate Lane 2 : : Liver (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-VPRBP antibody - ab177097 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VPRBP antibody (ab177097) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : PC3 cell lysateLane 4 : HEK293 cell lysate

Anti-VPRBP antibody - ab134339 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VPRBP antibody (ab134339) at 2 µg/mlLane 1 : Human brain tissue lysateLane 2 : Human brain tissue lysate with immunizing peptideSecondarygoat anti-rabbit Ig HRP HRP developed using the ECL technique

Anti-VPRBP antibody [EPR16012] - ab202587 from Abcam

Prices $99.00, $347.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16012
All lanes : Anti-VPRBP antibody [EPR16012] (ab202587) at 1/2000 dilutionLane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysateLane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Anti-VPRBP Antibody (aa25-75) - LS-C288664 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-VPRBP Antibody (aa1457-1507) - LS-C288665 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

VprBP Antibody - NBP2-27203 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: VprBP Antibody [NBP2-27203] - analysis of VPRBP using VPRBP antibody. Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of VPRBP antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

VprBP Antibody - NBP1-05952 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VprBP Antibody [NBP1-05952] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VprBP antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. VprBP was also immunoprecipitated by rabbit anti-VPRBP antibody NBP1-05951, which recognizes a downstream epitope.

VprBP Antibody - NBP1-05953 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

VprBP Antibody - NBP1-85284 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: VprBP Antibody [NBP1-85284] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

CAF1p55 Polyclonal Antibody - bs-9393R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-VPRBP antibody produced in rabbit - HPA036142 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-VPRBP: <B>Cat. No. HPA036142</B>: Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

VPRBP antibody - 70R-21260 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

VPRBP Antibody - abx036610 from Abbexa

Host Rabbit
Clonality Polyclonal

VPRBP antibody - 11612-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Hela cell were subjected to SDS PAGE followed by western blot with 11612-1-AP(VPRBP Antibody) at dilution of 1:1000

VPRBP antibody - 11612-1-AP from Proteintech Group

Prices $279.00
Sizes con: 25 μg/150μl
Host Rabbit
Clonality Polyclonal
HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP(VPRBP antibody) at dilution of 1:500

VprBP Antibody - 39965 from Signalway Antibody

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

VPRBP Antibody - E307108 from Enogene

Prices $275.00
Sizes 100ug/200ul

VprBP Antibody - A301-887A from Bethyl Labs

Prices $99.00, $229.00, $339.00
Sizes 20 µl (2 blots), 100 µl (10 blots), 100 µl (0.2 mg/ml)
Host Rabbit
Clonality Polyclonal

VprBP Antibody - A301-888A from Bethyl Labs

Prices $99.00, $229.00, $339.00
Sizes 20 µl (2 blots), 100 µl (10 blots), 100 µl (0.2 mg/ml)
Host Rabbit
Clonality Polyclonal
Search more