EIF3F

Gene Symbol EIF3F
Entrez Gene 8665
Alt Symbol EIF3S5, eIF3-p47
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 3, subunit F
Other Description deubiquitinating enzyme eIF3f|eIF-3-epsilon|eIF3-epsilon|eukaryotic translation initiation factor 3 subunit F|eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD)|eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
Swissprots A8K0S2 Q6IB45 Q8N978 O00303
Accessions EAW68637 O00303 AK027739 AK093511 BAG52733 AK095574 BAC04577 AK289637 BAF82326 AK291354 BAF84043 AK293826 BAG57230 AK297621 BAG59997 BC000490 AAH00490 BC073753 BT006894 AAP35540 CB141463 CD050033 CR456959 CAG33240 DQ890766 ABM81692 HQ448263 ADQ32743 U94855 AAD03467 NM_003754 NP_003745
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. {ECO:0000255|HAMAP- Rule:MF_03005, ECO:0000269|PubMed:21124883}.
Subcellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03005}.
Top Pathways RNA transport

eIF3ε (H-20) - sc-30247 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF3ε (H-20): sc-30247. Western blot analysis of eIF3ε expression in non-transfected: sc-117752 (A) and human eIF3ε transfected: sc-371433 (B) 293T whole cell lysates.

eIF3ε (K-15) - sc-30248 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-EIF3F antibody - ab64177 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab64177 at 2.5µg/ml staining EIF3F in human brain cerebellum by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.

Anti-EIF3F antibody - ab155475 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-EIF3F antibody (ab155475) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-EIF3F antibody - ab74568 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EIF3F antibody (ab74568) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from COLO205 cellsLane 3 : Extracts from COLO205 cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-EIF3F antibody - ab167263 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-EIF3F antibody (ab167263) at 1/500 dilution + Human colon tissue lysate at 25 µgdeveloped using the ECL technique

Anti-EIF3S5 / EIF3F Antibody (aa93-123) - LS-C167694 from LifeSpan Bioscience

Prices $250.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF3S5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Anti-EIF3S5 / EIF3F Antibody (aa288-305) - LS-C148960 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-EIF3S5 / EIF3F Antibody - LS-C307371 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-EIF3S5 / EIF3F Antibody (Internal) - LS-C345791 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-EIF3S5 / EIF3F Antibody (aa279-328) - LS-C145579 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EIF3F antibody LS-C145579 Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.

Anti-EIF3S5 / EIF3F Antibody (aa97-146) - LS-C145578 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EIF3F antibody LS-C145578 Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.

Anti-EIF3S5 / EIF3F Antibody (aa81-130) - LS-C119967 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human brain tissue, using EIF3F Antibody. The picture on the right is treated with the synthesized peptide.

Anti-EIF3S5 / EIF3F Antibody (aa307-357) - LS-C289712 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Anti-EIF3S5 / EIF3F Antibody (Val116) - LS-C177451 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of eIF3 (V116) pAb in paraffin-embedded human brain tissue.

Anti-EIF3S5 / EIF3F Antibody (aa114-125) IHC-plus™ - LS-B366 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-EIF3F antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B366 concentration 5 ug/ml.

EIF3F Antibody - NBP1-77997 from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

EIF3F Antibody - NBP2-16299 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3F Antibody [NBP2-16299] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:1000.

EIF3F Antibody - H00008665-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: EIF3F Antibody [H00008665-B01] - EIF3S5 MaxPab polyclonal antibody (B01), Lot # 07297WULz. Western Blot analysis of EIF3S5 expression in human spleen.

EIF3F Antibody - H00008665-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: EIF3F Antibody [H00008665-B01P] - Analysis of EIF3S5 expression in human spleen.

EIF3F Antibody - H00008665-B02 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: EIF3F Antibody [H00008665-B02] - Western Blot analysis of EIF3S5 expression in human colon.

EIF3F Antibody - H00008665-B02P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: EIF3F Antibody [H00008665-B02P] - Analysis of EIF3S5 expression in human colon.

EIF3F Antibody - NBP1-50037 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3F Antibody [NBP1-50037] - Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3F, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

EIF3F Antibody - H00008665-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: EIF3F Antibody [H00008665-A01] - EIF3S5 polyclonal antibody (A01), Lot # 070109QCSb Western Blot analysis of EIF3S5 expression in PC-12 ( Cat # L012V1 ).

eIF3F Polyclonal Antibody - bs-3841R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded human colon labeled with Rabbit Anti eIF3F Polyclonal Antibody, Unconjugated (bs-3841R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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