NIFK

Gene Symbol NIFK
Entrez Gene 84365
Alt Symbol MKI67IP, Nopp34
Species Human
Gene Type protein-coding
Description nucleolar protein interacting with the FHA domain of MKI67
Other Description MKI67 (FHA domain) interacting nucleolar phosphoprotein|MKI67 FHA domain-interacting nucleolar phosphoprotein|hNIFK|nucleolar phosphoprotein Nopp34|nucleolar protein interacting with the FHA domain of pKi-67
Swissprots Q9BYG3 Q8TB66 Q96ED4 A8K788
Accessions AAY14830 EAW95259 EAW95260 EAW95261 Q9BYG3 AB044971 BAB41210 AK291903 BAF84592 AK299814 BAG61686 AK311033 BC012457 AAH12457 BC022990 AAH22990 BC024238 AAH24238 BE671589 DA570244 DQ891678 ABM82604 DQ894863 ABM85789 NM_032390 NP_115766
Subcellular Location Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67.

NIFK (T-14) - sc-107819 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NIFK (T-14): sc-107819. Western blot analysis of NSE1 expression in HeLa whole cell lysate.

NIFK (H-63) - sc-292761 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

NIFK (18E148) - sc-52904 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
NIFK (18E148): sc-52904. Western blot analysis of NIFK expression in Raji whole cell lysate (A) and BJAB (B), Ramos (C) and HeLa (D) nuclear extracts.

Anti-NIFK (phospho T234) antibody - ab61110 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61110 at a 1/50-1/100 dilution, staining human NIFK in brain tissue with (+) or without (-) immunising phospopeptide, using Immunohistochemistry, Paraffin embedded tissue

Anti-NIFK antibody - ab61203 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61203 at 1/50 dilution staining NIFK in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.

Anti-NIFK antibody - ab89642 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-NIFK antibody (ab89642) at 1 µg/ml + human kidney tissue lysate at 50 µg

Anti-NIFK antibody [18E148] - ab13880 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 18E148
Overlay histogram showing Ramos cells stained with ab13880 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13880, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NIFK / MKI67IP Antibody (aa232-260, AP) - LS-C263186 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIFK / MKI67IP Antibody (aa232-260, APC) - LS-C263187 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIFK / MKI67IP Antibody (aa232-260, FITC) - LS-C263190 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIFK / MKI67IP Antibody (aa100-200, clone 18E148) - LS-C538 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 18E148
Western blot of NIFK in 30 ugs of Ramos cell lysate using antibody at 0.5 ug/ml.

Anti-NIFK / MKI67IP Antibody (aa232-260, Biotin) - LS-C263188 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIFK / MKI67IP Antibody (aa232-260, Azide-free, HRP) - LS-C263193 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIFK / MKI67IP Antibody (aa232-260, PE) - LS-C263192 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIFK / MKI67IP Antibody (aa200-249) - LS-C117983 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human brain tissue, using NIFK (Ab-234) Antibody. The picture on the right is treated with the synthesized peptide.

Anti-NIFK / MKI67IP Antibody - LS-C309008 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-NIFK / MKI67IP Antibody (aa232-260) - LS-C329831 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NIFK / MKI67IP Antibody (aa75-90) - LS-C748 from LifeSpan Bioscience

Prices $335.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Western blot of NIFK in 30 ugs of NIH3T3 cell lysate using antibody at 1:1000.

Anti-NIFK / MKI67IP Antibody (Asp229) - LS-C176544 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of NIFK (D229) pAb in paraffin-embedded human lung carcinoma tissue.

Anti-NIFK / MKI67IP Antibody (aa232-260) - LS-C157887 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MKI67IP Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The MKI67IP antibody detected the MKI67IP protein (arrow).

Anti-NIFK / MKI67IP Antibody (phospho-Thr234) - LS-C199323 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human brain, using NIFK (Phospho-Thr234) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-NIFK / MKI67IP Antibody (aa144-160) - LS-C707 from LifeSpan Bioscience

Prices $335.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
NIFK KI67ip -immunostaining in adipocytes of human Carcinomatous Breast tissue using antibody at 1:500 dilution.

Anti-NIFK / MKI67IP Antibody (aa243-293) - LS-C290692 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human MKI67IP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-MKI67IP antibody used for IP at 6 ug/mg lysate. MKI67IP was also immunoprecipitated by rabbit anti-MKI67IP antibody BL14642. For blotting immunoprecipitated MKI67IP, was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-NIFK / MKI67IP Antibody (aa140-189) IHC-plus™ - LS-B1546 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MKI67IP antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1546 concentration 5 ug/ml.

NIFK Antibody (CL2240) - NBP2-36749 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CL2240
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