CYP46A1

Gene Symbol CYP46A1
Entrez Gene 10858
Alt Symbol CP46, CYP46
Species Human
Gene Type protein-coding
Description cytochrome P450, family 46, subfamily A, polypeptide 1
Other Description CH24H|cholesterol 24-hydroxylase|cytochrome P450 46A1|cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
Swissprots Q9Y6A2 E7EQG9 Q8N2B0 B4DHP8
Accessions EAW81678 Q9Y6A2 AB209749 BAD92986 AF094480 AAD41244 AK090886 BAC03539 AK295216 BAG58210 BC022539 AAH22539 XM_005267274 XP_005267331 XM_011536364 XP_011534666 XM_011536365 XP_011534667 NM_006668 NP_006659
Function Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. Has also activity with xenobiotic compounds, such as clotrimazole. {ECO:0000269|PubMed:18621681, ECO:0000269|PubMed:20667828}.
Subcellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
Tissue Specificity Expressed in brain. The mRNA was broadly distributed with higher levels in gray matter zones and lower levels in regions rich in white matter. Not detected in fetal sample but its expression increases linearly with age.
Top Pathways Primary bile acid biosynthesis

CYP46 (V-17) - sc-74391 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CYP46 (V-17): sc-74391. Immunofluorescence staining of formalin-fixed HepG2 cells showing cytoplasmic localization.

CYP46 (1F11) - sc-136148 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

CYP46 (H-121) - sc-368609 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-CYP46 antibody - ab198889 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CYP46 antibody (ab198889) at 1/200 dilution + Human lymphoma tissue lysate at 40 µg

Anti-CYP46 antibody - ab126967 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-CYP46 antibody (ab126967) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

Anti-CYP46 antibody - ab82814 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab82814 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab82814 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CYP46 antibody - ab56784 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56784 CYP46 antibody at 1ug/ml.

Anti-CYP46A1 / CYP46 Antibody (C-Terminus) - LS-C56817 from LifeSpan Bioscience

Host Goat
Clonality Polyclonal

Anti-CYP46A1 / CYP46 Antibody (aa201-300) - LS-C125428 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-CYP46A1 / CYP46 Antibody (aa201-300) - LS-C307844 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-CYP46A1 / CYP46 Antibody - LS-C311077 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CYP46A1 / CYP46 Antibody (aa396-445) - LS-C80601 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-CYP46A1 / CYP46 Antibody - LS-C155427 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE CYP46A1 antibody diluted at 1:1000

Anti-CYP46A1 / CYP46 Antibody IHC-plus™ - LS-B279 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-CYP46A1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B279 dilution 1:500.

CYP46A1 Antibody - NB400-140 from Novus Biologicals

Prices $99.00, $279.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140.

CYP46A1 Antibody - NBP1-85362 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CYP46A1 Antibody [NBP1-85362] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)<br/>Lane 5: Human liver tissue

CYP46A1 Antibody - NBP2-16067 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CYP46A1 Antibody [NBP2-16067] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.

CYP46A1 Antibody - NBP2-16066 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CYP46A1 Antibody [NBP2-16066] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.

CYP46A1 Antibody (1A11) - H00010858-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A11

CYP46A1 Antibody (2B5) - H00010858-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B5
Sandwich ELISA: CYP46A1 Antibody (2B5) [H00010858-M02] - Detection limit for recombinant GST tagged CYP46A1 is 0.03 ng/ml as a capture antibody.

CYP46A1 Antibody - H00010858-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: CYP46A1 Antibody [H00010858-D01P] - Analysis of CYP46A1 expression in transfected 293T cell line by CYP46A1 polyclonal antibody.Lane 1: CYP46A1 transfected lysate(56.80 KDa).Lane 2: Non-transfected lysate.

Monoclonal Anti-CYP46A1 antibody produced in mouse - WH0010858M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A11
<B>Immunoblotting</B><BR/>Monoclonal Anti-CYP46A1, <B>Cat. No. WH0010858M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~37.11 kDa using immunogen protein lysate.

Anti-CYP46A1 antibody produced in rabbit - SAB2100523 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CYP46A1: <B>Cat. No.SAB2100523</B>: CYP46A1 in HepG2 cell lysate with CYP46A1 antibody at 1.0 μg/mL.

Monoclonal Anti-CYP46A1 antibody produced in mouse - SAB1404872 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B5
<B>Immunoblotting</B><BR/>Monoclonal Anti-CYP46A1: <B>Cat. No. SAB1404872</B>: Antibody concentration: 1 μg/mL. Specific band of ~37.1 kDa using immunogen protein lysate.

Anti-CYP46A1 antibody produced in rabbit - HPA040702 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CYP46A1: <B>Cat. No. HPA040702</B>: Immunohistochemical staining of human gall bladder shows strong cytoplasmic and membranous positivity in glandular cells.
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