Gene Symbol | POLR1A |
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Entrez Gene | 25885 |
Alt Symbol | A190, RPA1, RPA194, RPO1-4, RPO14 |
Species | Human |
Gene Type | protein-coding |
Description | polymerase (RNA) I polypeptide A, 194kDa |
Other Description | DNA-directed RNA polymerase I largest subunit|DNA-directed RNA polymerase I subunit A|DNA-directed RNA polymerase I subunit A1|DNA-directed RNA polymerase I subunit RPA1|RNA polymerase I 194 kDa subunit|RNA polymerase I subunit A1 |
Swissprots | Q0VG05 Q9UFT9 B7Z7T0 O95602 D6W5M0 Q9UEH0 |
Accessions | AAY24277 EAW99467 EAW99468 EAW99469 O95602 AK025568 BAB15171 AK294447 BAH11770 AK302458 BAH13716 AK304131 BAH14113 AK307490 AL117467 CAB55942 BC017115 AAH17115 BC073968 BC117173 AAI17174 BC126303 AAI26304 BC143345 AAI43346 BP363163 U33460 AAC99959 XM_006711983 XP_006712046 NM_015425 NP_056240 |
Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). {ECO:0000250}. |
Subcellular Location | Nucleus, nucleolus {ECO:0000250}. |
Top Pathways | Purine metabolism, RNA polymerase, Pyrimidine metabolism |
RPA194 CRISPR/Cas9 KO Plasmid (h) - sc-400816 from Santa Cruz Biotechnology
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RPA194 HDR Plasmid (h) - sc-400816-HDR from Santa Cruz Biotechnology
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RPA194 Double Nickase Plasmid (h) - sc-400816-NIC from Santa Cruz Biotechnology
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RPA194 Double Nickase Plasmid (h2) - sc-400816-NIC-2 from Santa Cruz Biotechnology
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RPA194 CRISPR Activation Plasmid (h) - sc-400816-ACT from Santa Cruz Biotechnology
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RPA194 CRISPR Activation Plasmid (h2) - sc-400816-ACT-2 from Santa Cruz Biotechnology
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RPA194 Lentiviral Activation Particles (h) - sc-400816-LAC from Santa Cruz Biotechnology
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RPA194 Lentiviral Activation Particles (h2) - sc-400816-LAC-2 from Santa Cruz Biotechnology
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RPA194 siRNA (h) - sc-38244 from Santa Cruz Biotechnology
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RPA194 shRNA Plasmid (h) - sc-38244-SH from Santa Cruz Biotechnology
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RPA194 shRNA (h) Lentiviral Particles - sc-38244-V from Santa Cruz Biotechnology
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RPA70 RNAi - H00006117-R01 from Novus Biologicals
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MISSION® esiRNA - EHU005201 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17106 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17106 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR13075 from Sigma-Aldrich
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POLR1A siRNA (Human) - CRH8563 from Cohesion Biosciences
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POLR1A RNAi - H00025885-R01 from Novus Biologicals
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shRNA set against Human POLR1A (NM_015425.3) - SHH379320 from Creative biogene
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shRNA set against Human POLR1A(NM_015425.3) - SHR127290 from Creative biogene
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POLR1A shRNA Plasmid (Human) - CHH8563 from Cohesion Biosciences
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POLR1A miRNA 3'UTR clone - MiUTR1H-08070 from Creative biogene
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