PMS2

Gene Symbol PMS2
Entrez Gene 5395
Alt Symbol HNPCC4, PMS2CL, PMSL2
Species Human
Gene Type protein-coding
Description PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
Other Description DNA mismatch repair protein PMS2|H_DJ0042M02.9|PMS1 protein homolog 2|mismatch repair endonuclease PMS2
Swissprots P54278 B2R610 Q75MR2 Q5FBX2 Q5FBX1 Q52LH6 Q5FBW9
Accessions AAA63923 AAS00390 ABQ59090 EAW55055 P54278 AB103082 BAD89425 AB103083 BAD89426 AB103085 BAD89428 AB103086 BAD89429 AB103087 BAD89430 AI433648 AK125607 AK294661 BAG57831 AK312390 BAG35307 BC008400 BC031832 BC093921 AAH93921 BC143397 BM669686 BP251389 BQ050630 BX537558 CAD97782 CR981250 DA774159 DB527312 U14658 AAA50390 XM_006715742 XP_006715805 XM_006715744 XP_006715807 XM_011515427 XP_011513729 XM_011515428 XP_011513730 XM_011515429 XP_011513731 XM_011515430 XP_011513732 NM_000535 NP_000526 NR_003085
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in ca
Subcellular Location Nucleus.
Top Pathways Mismatch repair, Fanconi anemia pathway

PMS2 (B-3) - sc-25315 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PMS2 (B-3): sc-25315. Western blot analysis of PMS2 expression in HeLa whole cell lysate.

PMS2 (C-20) - sc-618 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PMS2 (C-20): sc-618. Western blot analysis of PMS2 expression in HeLa (A) and A-431 (B) nuclear extracts.

PMS2 (E-19) - sc-617 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PMS2 (E-19): sc-617. Western blot analysis of PMS2 expression in Jurkat (A) and NIH/3T3 (B) nuclear extracts.

PMS2 (H-300) - sc-11440 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PMS2 (H-300): sc-11440. Western blot analysis of PMS2 expression in HeLa nuclear extract.

Anti-PMS2 Antibody (aa461-510) - LS-C119589 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa cells, using PMS2 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-PMS2 Antibody (aa611-660) - LS-C111807 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PMS2 antibody LS-C111807 Western blot of HepG2 cell lysate.

Anti-PMS2 Antibody (aa125-174) IHC-plus™ - LS-B5688 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PMS2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5688 concentration 10 ug/ml.

Anti-PMS2 Antibody (clone 4G4) - LS-C197934 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4G4
Detection limit for recombinant GST tagged PMS2 is approximately 3 ng/ml as a capture antibody.

Anti-PMS2 Antibody (clone EP51) - LS-C312146 from LifeSpan Bioscience

Prices $255.00, $405.00, $680.00
Sizes 100 µl, 500 µl, 1 ml
Host Rabbit
Clonality Monoclonal
Clone EP51
IHC of PMS2 on FFPE Colon Carcinoma tissue.

Anti-PMS2 Antibody (Asp483) - LS-C177136 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of PMS2 (D483) pAb in extracts from HeLa cells.

Anti-PMS2 Antibody (clone EP51, Ready-to-Use) - LS-C348570 from LifeSpan Bioscience

Prices $315.00, $435.00, $665.00
Sizes 3 ml, 7 ml, 15 ml
Host Rabbit
Clonality Monoclonal
Clone EP51
IHC of PMS2 on FFPE Colon Carcinoma tissue.

Anti-PMS2 Antibody - LS-C346151 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of U20S cell using PMS2 antibody.

Anti-PMS2 Antibody (Internal, clone EPR3947) - LS-C137741 from LifeSpan Bioscience

Prices $490.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3947
IHC of paraffin-embedded human colonic adenocarcinoma using anti-PMS2 antibody.

Anti-PMS2 Antibody (aa623-639, clone 163C1251) IHC-plus™ - LS-B1980 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 163C1251
Anti-PMS2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1980 concentration 5 ug/ml.

Anti-PMS2 Antibody (clone 4B7) - LS-C338148 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4B7
IHC of paraffin-embedded Human pancreas tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-PMS2 Antibody (clone 2G5) - LS-C338149 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G5
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-PMS2 Antibody - LS-C48436 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PMS2 Antibody (Agarose) - LS-C92003 from LifeSpan Bioscience

Prices $635.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PMS2 Antibody (aa1-50) - LS-C287022 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PMS2 Antibody (aa1-50, Agarose) - LS-C291116 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PMS2 Antibody (aa58-81, clone 349.29.5.2) - LS-C59521 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 349.29.5.2
Anti-PMS2 Antibody - Western Blot. Western blot analysis is shown using Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5,10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1000 dilution of the antibody at room temperature followed by washing. A 1:20000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemicals SuperSignal substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kD corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results. Personal communication Morphotek Inc.

Anti-PMS2 Antibody (aa434-483) - LS-C200503 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HUVEC cells using PMS2 antibody.

PMS2 Antibody (163C1251) - NB100-56554 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 163C1251
Western Blot: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.

PMS2 Antibody (EPR3947) - NBP1-95277 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3947
Western Blot: PMS2 Antibody (EPR3947) [NBP1-95277] - (A) Jurkat, (B) HeLa, © SH-SY5Y and (D) SKBR-3 cell lysates.

PMS2 Antibody - H00005395-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: PMS2 Antibody [H00005395-B01] - Western Blot analysis of PMS2 expression in human spleen.
Search more