PIWIL3

Gene Symbol	PIWIL3
Entrez Gene	440822
Alt Symbol	HIWI3
Species	Human
Gene Type	protein-coding
Description	piwi-like RNA-mediated gene silencing 3
Other Description	piwi-like 3\|piwi-like protein 3
Swissprots	Q7Z3Z3
Accessions	EAW59679 Q7Z3Z3 AB079368 BAC81343 AK302409 BAG63719 AK315806 BC144693 BC144696 BC167797 AAI67797 NM_001008496 NP_001008496 NM_001255975 NP_001242904 NR_045648 NR_045649
Function	May play a role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). {ECO:0000250}.
Subcellular Location	Cytoplasm. Note=Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. {ECO:0000250}.
Tissue Specificity	Expressed in testis. {ECO:0000269\|PubMed:12906857}.
Top Pathways	Dorso-ventral axis formation

PIWIL3 (C-3) - sc-398779 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

PIWIL3 (C-3): sc-398779. Western blot analysis of PIWIL3 expression in NTERA-2 cl.D1 (A), Hs 181 Tes (B), NIH/3T3 (C) and HeLa (D) whole cell lysates.

PIWIL3 (N-20) - sc-68718 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

PIWIL3 (H-90) - sc-68931 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-PIWIL3 antibody - ab93709 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ICC/IF image of ab93709 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93709 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PIWIL3 antibody - ab77088 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-PIWIL3 antibody (ab77088) at 5 µg/mlLane 1 : PIWIL3 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-PIWIL3 antibody - ab39379 from Abcam

Prices	$242.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PIWIL3 antibody - C-terminal - ab200146 from Abcam

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-PIWIL3 antibody - C-terminal (ab200146) at 1/1000 dilutionLane 1 : Total cell extracts from HeLa cellsLane 2 : Total cell extracts from Jurkat cells

Anti-PIWIL3 Antibody (Internal) - LS-C137071 from LifeSpan Bioscience

Prices	$255.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PIWIL3 Antibody (N-Terminus) - LS-C115923 from LifeSpan Bioscience

Prices	$345.00, $1,035.00
Sizes	100 µg, 500 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence of PIWI-L3 in 3T3 cells with PIWI-L3 antibody at 20 ug/ml.

PIWIL3 Antibody - NBP2-31855 from Novus Biologicals

Prices	$129.00, $419.00
Sizes	25 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: PIWIL3 Antibody [NBP2-31855] - Staining of human stomach, upper shows strong membranous positivity in glandular cells.

PIWIL3 Antibody - NBP1-76490 from Novus Biologicals

Prices	$329.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: PIWIL3 Antibody [NBP1-76490] - 3T3 cell lysate with PIWI-L3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

PIWIL3 Antibody - H00440822-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: PIWIL3 Antibody [H00440822-B01P] - Analysis of PIWIL3 expression in transfected 293T cell line by PIWIL3 polyclonal antibody. Lane 1: PIWIL3 transfected lysate(97.02 KDa). Lane 2: Non-transfected lysate.

Rabbit Anti-PIWIL3 Polyclonal Antibody - bs-6348R from Bioss

Host	Rabbit
Clonality	Polyclonal

Anti-PIWIL3 (N-terminal) antibody produced in rabbit - SAB4200150 from Sigma-Aldrich

Prices	$55.60, $322.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Formalin-fixed, paraffin-embedded tissue sections of human Colon Carcinoma stained with 10 μg/mL Anti-PIWIL3 (N-terminal) (<B>Cat. No. SAB4200150</B>). A. ×40; B. ×20

Anti-PIWIL3 antibody produced in mouse - SAB1408537 from Sigma-Aldrich

Host	Mouse
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-PIWIL3: <B>Cat. No. SAB1408537</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~97 kDa using PIWIL3 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-PIWI-L3 antibody produced in rabbit - SAB3500579 from Sigma-Aldrich

Prices	$346.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-PIWI-L3: <B>Cat. No. SAB3500579</B>: PIWI-L3 in 3T3 cell lysate with PIWIL3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

PIWIL3 antibody - orb77272 from Biorbyt

Prices	$425.00
Sizes	100 μg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of formalin-fixed paraffin embedded testis showing nuclear staining using PIWIL3 antibody

PIWIL3 Antibody - abx037378 from Abbexa

Host	Rabbit
Clonality	Polyclonal

PIWIL3 (N-term) antibody - AP26293PU-N from Acris Antibodies

Prices	$340.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of PIWI-L3 in 3T3 cell lysate with PIWI-L3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.

PIWIL3 antibody - H00440822-B01P from Acris Antibodies

Prices	$360.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot analysis of PIWIL3 expression in transfected 293T cell line (H00440822-T01) by PIWIL3 MaxPab polyclonal antibody. Lane 1: PIWIL3 transfected lysate(97.02 KDa). Lane 2: Non-transfected lysate.

PIWIL3 antibody - H00440822-B02P from Acris Antibodies

Prices	$1,530.00
Sizes	500 µg
Host	Mouse
Clonality	Polyclonal

PIWI-L3 Antibody - 6033 from ProSci

Prices	$79.00, $325.00
Sizes	20 µg, 100 µg
Host	Rabbit
Clonality	Polyclonal

PIWI-L3 Antibody - 254376 from ABBIOTEC

Prices	$292.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

PIWIL3 Antibody - 37360 from Signalway Antibody

Prices	$279.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #37360 at dilution 1/20.

PIWI-L3 Antibody - 25211 from Signalway Antibody

Prices	$390.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of PIWI-L3 in 3T3 cell lysate with PIWI-L3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

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