GPI

Gene Symbol GPI
Entrez Gene 2821
Alt Symbol AMF, GNPI, NLK, PGI, PHI, SA-36, SA36
Species Human
Gene Type protein-coding
Description glucose-6-phosphate isomerase
Other Description autocrine motility factor|glucose phosphate isomerase|hexose monophosphate isomerase|hexosephosphate isomerase|neuroleukin|oxoisomerase|phosphoglucose isomerase|phosphohexomutase|phosphohexose isomerase|phosphosaccharomutase|sperm antigen 36|sperm antigen-36
Swissprots Q9BSK5 Q9BRD3 Q9UHE6 B4DG39 P06744
Accessions AAP72966 P06744 AB209575 BAD92812 AK025421 AK129884 AK130102 AK293446 BAG56947 AK294396 BAG57650 AK301103 BAG62702 BC004982 AAH04982 BC006342 BM664761 BX641158 CR745343 DC299841 DC342043 DQ892924 ABM83850 DQ896173 ABM87172 FY213211 K03515 AAA36368 X16539 XM_005258764 XP_005258821 XM_006723148 XP_006723211 XM_011526754 XP_011525056 XM_011526755 XP_011525057 NM_000175 NP_000166 NM_001184722 NP_001171651 NM_001289789 NP_001276718 NM_001289790 NP_001276719
Function Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. {ECO:0000269|PubMed:11004567, ECO:0000269|PubMed:11437381}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11437381}. Secreted {ECO:0000269|PubMed:11437381}.
Top Pathways Glycolysis / Gluconeogenesis, Pentose phosphate pathway, Amino sugar and nucleotide sugar metabolism, Carbon metabolism, Starch and sucrose metabolism

GPI (A-5) - sc-398381 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPI (A-5): sc-398381. Western blot analysis of GPI expression in HEK293 (A), HeLa (B), RT-4 (C) and U-251-MG (D) whole cell lysates.

GPI (E-4) - sc-398382 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPI (E-4): sc-398382. Western blot analysis of GPI expression in HEK293 (A), HeLa (B), RT-4 (C) and U-251-MG (D) whole cell lysates.

GPI (H-10) - sc-365066 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPI (H-10): sc-365066. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

GPI (1B7D7) - sc-130067 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPI (1B7D7): sc-130067. Western blot analysis of GPI expression in Hep G2 (A) and SMMC-7721 (B) whole cell lysates.

GPI (H-300) - sc-33777 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GPI (H-300): sc-33777. Immuno?fluorescence staining of methanol-fixed HeLa cells showing cytoplasmic local?ization.

GPI (H-9) - sc-271459 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPI (H-9): sc-271459. Western blot analysis of GPI expression in HEK293 (A) and Hep G2 (B) whole cell lysates.

GPI (K-16) - sc-30392 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GPI (K-16): sc-30392. Western blot analysis of GPI expression in non-transfected: sc-117752 (A) and human GPI transfected: sc-170846 (B) 293T whole cell lysates.

Anti-Glucose 6 phosphate isomerase antibody - ab86950 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Glucose 6 phosphate isomerase antibody (ab86950) at 1 µg/mlLane 1 : Heart (Rat) Tissue Lysate Lane 2 : Lung (Rat) Tissue LysateLane 3 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Glucose 6 phosphate isomerase antibody - ab68643 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab68643 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68643, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293 and MCF7 cells at 5µg/ml.

Anti-Glucose 6 phosphate isomerase antibody - ab110051 from Abcam

Prices $384.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-Glucose 6 phosphate isomerase antibody (ab110051) at 0.01 µg/ml + Human Heart lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-Glucose 6 phosphate isomerase antibody - ab89596 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Glucose 6 phosphate isomerase antibody (ab89596) at 1 µg/ml + Human liver tissue lysate at 50 µg

Anti-Glucose 6 phosphate isomerase antibody - ab81466 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Glucose 6 phosphate isomerase antibody - ab99189 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Glucose 6 phosphate isomerase antibody (ab99189) at 0.3 µg/ml + Human heart lysate at 35 µgdeveloped using the ECL technique

Anti-Glucose 6 phosphate isomerase antibody [1B7D7] - ab66340 from Abcam

Prices $394.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B7D7
Overlay histogram showing HepG2 cells stained with ab66340 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66340, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Glucose 6 phosphate isomerase antibody [2D2] - ab118149 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D2
All lanes : Anti-Glucose 6 phosphate isomerase antibody [2D2] (ab118149) at 1/1000 dilutionLane 1 : Non-transfected HEK293T cell lysateLane 2 : Glucose 6 phosphate isomerase transfected HEK293T cell lysateLysates/proteins at 5 µg per lane.

Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)] - ab167394 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11663(B)
ab167394 showing +ve staining in Human normal brain.

Anti-Glucose 6 phosphate isomerase antibody - Carboxyterminal end - ab55973 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPI Antibody (aa445-473) - LS-C166610 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysates from Ramos cell line,mouse liver,rat liver tissue (from left to right),using GPI Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Anti-GPI Antibody (clone 4B11) IHC-plus™ - LS-B11078 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 4B11
Anti-GPI antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11078 concentration 10 ug/ml.

Anti-GPI Antibody (clone 1B7D7) IHC-plus™ - LS-B4092 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 1B7D7
Anti-GPI antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4092 dilution 1:200.

Anti-GPI Antibody (C-Terminus) - LS-C47061 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPI Antibody (Internal) - LS-C144981 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GPI Antibody - LS-C109500 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate). A: H1299. B: Raji. 7.5% SDS PAGE. GPI antibody diluted at 1:10000.

Anti-GPI Antibody (phospho-Ser185) - LS-C145086 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GPI Antibody (C-Terminus) - LS-C135916 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.
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