RAMP3

Gene Symbol RAMP3
Entrez Gene 10268
Alt Symbol -
Species Human
Gene Type protein-coding
Description receptor (G protein-coupled) activity modifying protein 3
Other Description CRLR activity-modifying protein 3|calcitonin receptor-like receptor activity modifying protein 3|calcitonin-receptor-like receptor activity-modifying protein 3|receptor (calcitonin) activity modifying protein 3|receptor activity modifying protein 3|receptor activity-modifying protein 3|receptor-activity-modifying protein 3
Swissprots Q7Z2Y1 O60896
Accessions BAN10197 BAN10198 EAL23743 EAW61048 EAW61049 EAW61050 O60896 AJ001016 CAA04474 AK128765 AK222469 BAD96189 AK311871 BAG34812 AY265459 AAP23300 BC022304 AAH22304 BC053852 AAH53852 BP347072 DQ891371 ABM82297 HQ448585 ADQ33063 XM_006715631 XP_006715694 NM_005856 NP_005847
Function Plays a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a GPER1-dependent manner. Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) and GPER1 to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. {ECO:0000269|PubMed:23674134, ECO:0000269|PubMed:9620797}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:23674134}; Single-pass type I membrane protein {ECO:0000269|PubMed:23674134}. Membrane {ECO:0000269|PubMed:23674134}; Single-pass type I membrane protein {ECO:0000269|PubMed:23674134}. Note=Moves from intracellular puncta to the plasma membrane in a RAMP3-dependent manner.
Tissue Specificity Strongly expressed in lung, breast, immune system and fetal tissues. {ECO:0000269|PubMed:9620797}.
Top Pathways Vascular smooth muscle contraction

RAMP3 (G-1) - sc-365313 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RAMP3 (G-1): sc-365313. Western blot analysis of RAMP3 expression in P23 whole cell lysate.

RAMP3 (C-20) - sc-8855 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

RAMP3 (N-15) - sc-8854 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RAMP3 (N-15): sc-8854. Western blot analysis of RAMP3 expression in non-transfected 293T: sc-117752 (A), human RAMP3 transfected 293T: sc-116550 (B) and P 23 (C) whole cell lysates.

RAMP3 (H-125) - sc-11381 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 antibody - ab197372 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue, labeling RAMP3 with ab197372 at 1/40 dilution. 

Anti-RAMP3 antibody - ab58660 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab58660 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58660, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RAMP3 antibody - ab123598 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RAMP3 antibody (ab123598) at 1/100 dilution + NCI-H292 cell line lysate at 35 µg

Anti-RAMP3 antibody - ab78017 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast tissue using ab78017 at 20µg/ml.

Anti-RAMP3 antibody - ab56684 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56684 RAMP3 antibody at 1ug/ml.

Anti-RAMP3 Antibody (C-Terminus) - LS-C9913 from LifeSpan Bioscience

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody (aa100-113) - LS-C148886 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody (aa90-118, AP) - LS-C267794 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody (aa90-118, APC) - LS-C267795 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody (aa90-118, Biotin) - LS-C267796 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody (aa90-118, FITC) - LS-C267797 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody (aa90-118, Azide-free, HRP) - LS-C267798 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody (aa90-118, PE) - LS-C267800 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody (aa89-118) - LS-C324516 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMP3 Antibody - LS-C334901 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using RAMP3 antibody.

Anti-RAMP3 Antibody (aa90-118) - LS-C162425 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RAMP3 Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The RAMP3 antibody detected the RAMP3 protein (arrow).

Anti-RAMP3 Antibody (N-Terminus) IHC-plus™ - LS-B1833 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RAMP3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1833 concentration 20 ug/ml.

Anti-RAMP3 Antibody (aa84-148) IHC-plus™ - LS-B11613 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Human Colon, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-RAMP3 Antibody (clone 1B4) - LS-C139128 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1B4

RAMP3 Antibody - AF4875 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RAMP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4875) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP3 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

RAMP3 Antibody - NBP1-39782 from Novus Biologicals

Prices $329.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: RAMP3 Antibody [NBP1-39782] - Analysis of anti-RAMP3 antibody with human breast at concentration 20 ug/ml.
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