DDAH2

Gene Symbol DDAH2
Entrez Gene 23564
Alt Symbol DDAH, DDAHII, G6a, HEL-S-277, NG30
Species Human
Gene Type protein-coding
Description dimethylarginine dimethylaminohydrolase 2
Other Description N(G),N(G)-dimethylarginine dimethylaminohydrolase 2|S-phase protein|dimethylargininase-2|dimethylarginine dimethylaminohydrolase II|epididymis secretory protein Li 277
Swissprots A2BEZ7 O95865
Accessions AAD18074 BAB63377 CAB46079 EAX03500 EAX03501 EAX03502 O95865 AA297871 AF070667 AAD20973 AF087894 AAP97193 AK026191 AK098671 AK310024 BC001435 AAH01435 BF338733 CB242623 EB387244 FJ224290 ACI45982 XM_011514448 XP_011512750 XM_011547196 XP_011545498 XM_011547610 XP_011545912 XM_011547847 XP_011546149 XM_011548016 XP_011546318 XM_011548205 XP_011546507 XM_011548389 XP_011546691 NM_001303007 NP_001289936 NM_001303008 NP_001289937 NM_013974 NP_039268
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. {ECO:0000269|PubMed:10493931}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:21898353}. Mitochondrion {ECO:0000269|PubMed:21898353}. Note=Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.
Tissue Specificity Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain. {ECO:0000269|PubMed:10493931}.

DDAH II (H-85) - sc-32859 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DDAH II (H-85): sc-32859. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.

DDAH II (C-19) - sc-26071 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDAH II (C-19): sc-26071. Western blot analysis of DDAH II expression in non-transfected: sc-117752 (A) and mouse DDAH II transfected: sc-119697 (B) 293T whole cell lysates.

DDAH II (N-20) - sc-26069 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDAH II (N-20): sc-26069. Immunofluorescence staining of methanol-fixed DU 145 cells showing cytoplasmic localization.

Anti-DDAH2 antibody - ab1383 from Abcam

Prices $385.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-DDAH2 antibody (ab1383) at 1 µg/ml + Human Heart extracts at 35 µg

Anti-DDAH2 antibody - ab167268 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DDAH2 antibody (ab167268) at 1 µg/mlLane 1 : DDAH2 transfected 293T cell line lysateLane 2 : Non transfected lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-DDAH2 antibody [EPR15508(B)] - ab184166 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15508(B)
Western blot analysis of immunoprecipitation pellet from Human fetal brain tissue lysate (lane 1) or a PBS control (lane 2) immunoprecipitated using ab184166 at 1/50 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Anti-DDAH2 Antibody (aa31-265, Biotin) - LS-C300196 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DDAH2 Antibody (aa31-265, FITC) - LS-C303134 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DDAH2 Antibody - LS-C307200 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-DDAH2 Antibody (aa273-285) - LS-C54711 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (1 ug/ml) of human heart extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-DDAH2 Antibody (aa31-265) - LS-C293692 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant DDAH2 / DDAH.

Anti-DDAH2 Antibody - LS-C334731 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using DDAH2 antibody.

Anti-DDAH2 Antibody (N-Terminus) IHC-plus™ - LS-B4001 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DDAH2 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4001 concentration 5 ug/ml.

DDAH2 Antibody - NBP1-58859 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DDAH2 Antibody [NBP1-58859] - Titration: 1 ug/ml Positive Control: Fetal Brain Lysate.

DDAH2 Antibody - NBP1-86092 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DDAH2 Antibody [NBP1-86092] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

DDAH2 Antibody - NB100-864 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: DDAH2 Antibody [NB100-864] - Analysis of DDAH2 in human Heart extracts using this antibody at 1 ug/ml.

DDAH2 Antibody - NBP2-16121 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DDAH2 Antibody [NBP2-16121] - Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE gel, diluted at 1:1000.

DDAH2 Antibody - H00023564-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: DDAH2 Antibody [H00023564-B01P] - Analysis of DDAH2 expression in transfected 293T cell line by DDAH2 polyclonal antibody. Lane 1: DDAH2 transfected lysate(31.46 KDa). Lane 2: Non-transfected lysate.

DDAH2 Antibody (3E3) - H00023564-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E3

DDAH2 Polyclonal Antibody - bs-2785R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat lung labeled with Anti-DDAH2 Polyclonal Antibody, Unconjugated (bs-2785R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Anti-DDAH2, (N-terminal) antibody produced in rabbit - SAB2103305 from Sigma-Aldrich

Prices $347.50
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DDAH2, (N-terminal): <B>Cat. No. SAB2103305</B>: Immunohistochemistry of DDAH2 in Human Uterus tissue with DDAH2 antibody at 5.0 μg/mL.

Anti-DDAH2 antibody produced in rabbit - HPA012509 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DDAH2: <B>Cat. No. HPA012509</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney shows strong cytoplasmic positivity in cells of tubules.

Anti-DDAH2 antibody produced in goat - SAB2500302 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-DDAH2: <B>Cat. No. SAB2500302</B>: staining (1 μg/mL) of human heart extracts (RIPA buffer, 35 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Monoclonal Anti-DDAH2 antibody produced in mouse - SAB1403152 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E3
<B>Immunoblotting</B><BR/>Monoclonal Anti-DDAH2: <B>Cat. No. SAB1403152</B>: Antibody concentration: 1 μg/mL. Specific band of ~57.5 kDa using immunogen protein lysate.

Anti-DDAH2 antibody produced in mouse - SAB1409034 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-DDAH2: <B>Cat. No. SAB1409034</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~31.5 kDa using DDAH2 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.
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