MMP9

Gene Symbol MMP9
Entrez Gene 4318
Alt Symbol CLG4B, GELB, MANDP2, MMP-9
Species Human
Gene Type protein-coding
Description matrix metallopeptidase 9
Other Description macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase-9|type V collagenase
Swissprots Q9UCL1 Q3LR70 P14780 Q8N725 Q9H4Z1 B2R7V9 Q9UDK2 Q9UCJ9
Accessions AAD37404 AAM97934 ABA03169 BAA20967 CAC07541 EAW75776 P14780 AK298246 BAH12743 AK301446 BAH13483 AK302530 BAH13736 AK303080 BAH13895 AK311648 AK313137 BAG35956 AK316145 BAH14516 AW468235 BC006093 AAH06093 CN288579 DQ896791 ABM87790 EU176415 ABW03866 HQ874462 AEA49029 J05070 AAA51539 NM_004994 NP_004985
Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. {ECO:0000269|PubMed:1480034}.
Subcellular Location Secreted, extracellular space, extracellular matrix {ECO:0000305}.
Tissue Specificity Produced by normal alveolar macrophages and granulocytes.
Top Pathways Leukocyte transendothelial migration, Bladder cancer, TNF signaling pathway, MicroRNAs in cancer, Estrogen signaling pathway

MMP-9 (D6O3H) XP ® Rabbit mAb - 13667 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6O3H
Western blot analysis of concentrated, serum-free cultured medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +), using MMP-9 (D6O3H) XP ® Rabbit mAb.

MMP-9 (G657) Antibody - 2270 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of partially purified, concentrated, serum free medium containing human MMP-9, using MMP-9 (G567) Antibody.

MMP-9 Antibody - 3852 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of partially purified, concentrated, serum-free medium containing human MMP-9, using MMP-9 Antibody.

MMP-9 (2C3) - sc-21733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MMP-9 (2C3): sc-21733. Western blot analysis of human recombinant MMP-9.

MMP-9 (6-6B) - sc-12759 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MMP-9 (6-6B): sc-12759. Western blot analysis of human recombinant MMP-9.

MMP-9 (7-11C) - sc-13520 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MMP-9 (7-11C): sc-13520. Western blot analysis of human recombinant MMP-9.

MMP-9 (E-11) - sc-393859 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MMP-9 (E-11): sc-393859. Western blot analysis of MMP-9 expression in AMJ2-C8 (A) and A-431 (B) whole cell lysates.

MMP-9 (C-20) - sc-6840 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MMP-9 (C-20): sc-6840. Western blot analysis of human recombinant MMP-9.

MMP-9 (M-17) - sc-6841 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MMP-9 (M-17): sc-6841. Western blot analysis of human recombinant MMP-9.

MMP-9 (H-129) - sc-10737 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Ac-MMP-9 (4A3) - sc-21736 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-MMP9 antibody - ab7299 from Abcam

Host Rabbit
Clonality Polyclonal
IHC image of MMP9 staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab7299, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MMP9 antibody - ab5707 from Abcam

Prices $379.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PMA stimulated A431 was probed with rabbit anti MMP9 at 1/500 (Lane A). These bands were abolished by blocking peptide (Lane B)

Anti-MMP9 antibody - ab73734 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MMP9 antibody (ab73734) at 1 µg/ml + Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-MMP9 antibody - ab137651 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MMP9 antibody (ab137651) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysate Lysates/proteins at 30 µg per lane.

Anti-MMP9 antibody - ab124513 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab124513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab124513 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MMP9 antibody - ab53018 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP9 antibody ab53018 1/50. The left hand image shows untreated tissue; right hand image shows tissue treated with the immunising peptide.

Anti-MMP9 antibody - ab38902 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Standard curve for MMP9 (Analyte: ab82955); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [SB15c] to MMP9 (ab51203) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP9 - Hinge region (ab38902) at 0.5µg/ml.

Anti-MMP9 antibody - ab74277 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab74277, at 1/100 dilution, staining MMP9 in formalin-fixed, paraffin-embedded human placenta tissue by immunohistochemistry.

Anti-MMP9 antibody - ab5936 from Abcam

Prices $372.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-MMP9 antibody - ab2167 from Abcam

Prices $370.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-MMP9 antibody - ab38899 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MMP9 antibody (ab38899)Lane 1 : Human MMP9Lane 2 : Cell media from human chondrosarcoma (untreated)

Anti-MMP9 antibody [15W2] - ab49478 from Abcam

Prices $378.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 15W2
ab49478 staining MMP9 in human oesophagus.Left panel: with primary antibody diluted at 1:1000. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-MMP9 antibody [2C3] - ab53296 from Abcam

Prices $376.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 2C3

Anti-MMP9 antibody [56-2A4] - ab58803 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 56-2A4
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