Anti-MMP9 antibody
| Name | Anti-MMP9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab73734 |
| Prices | $388.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F WB |
| Species Reactivities | Human, Rabbit, Horse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MMP9 |
| Description | Rabbit Polyclonal |
| Gene | MMP9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-MMP9 antibody (ab73734) at 1 µg/ml + Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
IHC image of MMP9 staining in human colon acrcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73734, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anti-MMP9 antibody (ab73734) at 1 µg + Human MMP9 full length protein (ab82955) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 2 minutes