UBE2B

Gene Symbol	UBE2B
Entrez Gene	7320
Alt Symbol	E2-17kDa, HHR6B, HR6B, RAD6B, UBC2
Species	Human
Gene Type	protein-coding
Description	ubiquitin-conjugating enzyme E2B
Other Description	E2 protein\|RAD6 homolog B\|ubiquitin carrier protein B\|ubiquitin-conjugating enzyme E2 B\|ubiquitin-conjugating enzyme E2-17 kDa\|ubiquitin-conjugating enzyme E2B (RAD6 homolog)\|ubiquitin-protein ligase B
Swissprots	D3DQA2 Q9D0J6 Q4PJ15 P23567 P63146 B2R503
Accessions	AAY68224 EAW62257 EAW62258 P63146 AK312012 BAG34950 BC001694 BC005979 AAH05979 BC008404 AAH08404 BC008470 AAH08470 BT007071 AAP35734 BU954901 CR407634 CAG28562 DA523817 DQ893113 ABM84039 DQ896387 ABM87386 M74525 AAA35982 T88933 X53251 CAA37339 NM_003337 NP_003328
Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys- 164'. May be involved in neurite outgrowth. {ECO:0000269\|PubMed:16337599, ECO:0000269\|PubMed:17108083, ECO:0000269\|PubMed:17130289, ECO:0000269\|PubMed:1717990, ECO:0000269\|PubMed:20061386}.
Subcellular Location	Cell membrane {ECO:0000250}. Nucleus {ECO:0000250}. Note=In peripheral neurons, expressed both at the plasma membrane and in nuclei. {ECO:0000250}.
Top Pathways	Ubiquitin mediated proteolysis

Anti-Ube2B antibody - ab199291 from Abcam

Prices	$376.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Ube2B antibody (ab199291) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : HepG2 cell lysateLane 4 : K562 cell lysateLane 5 : NIH 3T3 cell lysateLane 6 : 7721 cell lysate

Anti-Ube2B antibody - ab96848 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Ube2B antibody (ab96848) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-Ube2B antibody - ab135104 from Abcam

Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Ube2B antibody (ab135104) at 1/1000 dilutionLane 1 : Total cell extracts from HeLa Lane 2 : Total cell extracts from Mouse MEF

Anti-Ube2B antibody - ab92919 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-Ube2B antibody (ab92919) at 500 µg + Jurkat cell lysate at 50 µg

Anti-Ube2B antibody - ab67118 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-Ube2B antibody (ab67118) at 1/500 dilutionLane 1 : UBE2B transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Anti-Ube2B antibody [EPR7199] - ab128951 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7199

All lanes : Anti-Ube2B antibody [EPR7199] (ab128951) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HepG2 cell lysateLane 3 : A375 cell lysateLane 4 : K-562 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HR6B / UBE2B Antibody (C-Terminus, Biotin) - LS-C283916 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HR6B / UBE2B Antibody (C-Terminus, APC) - LS-C283917 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HR6B / UBE2B Antibody (C-Terminus, FITC) - LS-C283918 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HR6B / UBE2B Antibody (C-Terminus, Azide-free, HRP) - LS-C283919 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HR6B / UBE2B Antibody (C-Terminus, PE) - LS-C283920 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HR6B / UBE2B Antibody (Internal, clone EPR7199) - LS-C152449 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7199

IHC of paraffin-embedded human kidney tissue using anti-UBE2B antibody.

Anti-HR6B / UBE2B Antibody (C-Terminus) - LS-C328989 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HR6B / UBE2B Antibody (clone 4C3) - LS-C198393 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4C3

Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M06), clone 4C3.Lane 1: UBE2B transfected lysate(17.3 KDa).Lane 2: Non-transfected lysate.

Anti-HR6B / UBE2B Antibody - LS-C109312 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Sample (30 ug of whole cell lysate). A: Hela. 12% SDS PAGE. UBE2B antibody. UBE2B antibody diluted at 1:1000.

Anti-HR6B / UBE2B Antibody (clone 1E11) - LS-C338792 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1E11

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2B.

Anti-HR6B / UBE2B Antibody - LS-C334634 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using UBE2B antibody.

Anti-HR6B / UBE2B Antibody (aa117-146) - LS-C98024 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-HR6B / UBE2B Antibody (C-Terminus, AP) - LS-C283915 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

HR6B/UBE2B Antibody - NBP1-31293 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: HR6B/UBE2B Antibody [NBP1-31293] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells. Used at a dilution of 1:5000.

HR6B/UBE2B Antibody (4C3) - H00007320-M06 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4C3

Western Blot: HR6B/UBE2B Antibody (4C3) [H00007320-M06] - Analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M06), clone 4C3. Lane 1: UBE2B transfected lysatE (17.3 KDa). Lane 2: Non-transfected lysate.

HR6B/UBE2B Antibody - H00007320-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: Ube2B Antibody [H00007320-B01] - UBE2B MaxPab polyclonal antibody. Western Blot analysis of UBE2B expression in Jurkat.

HR6B/UBE2B Antibody - NBP2-20781 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: HR6B/UBE2B Antibody [NBP2-20781] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

HR6B/UBE2B Antibody - H00007320-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

HR6B/UBE2B Antibody (1F11) - H00007320-M13 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F11

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