Mouse Mus musculus



17 beta Estradiol ELISA Kit - ab108667 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 8.68 pg/ml
Range 20 pg/ml - 2000 pg/ml
17 beta Estradiol measured in biological fluids showing quantity (pg) per mL of tested sample

ACADM ELISA Kit - ab129734 from Abcam

Category ELISA Kit
Prices $448.00
Sizes 1 x 96 tests
Sensitivity 2 ng/ml
Range Sample specific recoverySample typeAverage %RangeSerum10495% - 118%Cell culture media10597% - 114%
Example of serially titrated ACADM protein in the assay working range.

ACE (CD143) Mouse ELISA Kit - ab155452 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity < 0.12 ng/ml
Range 30 ng/ml - 0.123 ng/ml
Representative standard curve using ab155452 - Assay Diluent A.

Aconitase 2 Profiling ELISA Kit - ab156028 from Abcam

Category ELISA Kit
Sensitivity <> 6 µg/ml
Range Sample specific recoverySample typeAverage %RangeSerum<>8370% - 101%Cell culture media<>11384% - 129%Extraction Buffer<>115100% - 121%
A dilution series of the lysate in 1X Incubation Buffer within the working range of the assay.

Activin A Mouse ELISA Kit - ab119569 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity < 10 pg/ml
Range 62.5 pg/ml - 4000 pg/ml
Representative Standard Curve using ab199569.

Activin A Rat ELISA Kit - ab193733 from Abcam

Category ELISA Kit
Prices $475.00
Sizes 1 x 96 tests
Sensitivity < 2 pg/ml
Range 2 pg/ml - 400 pg/ml
A typical standard curve using ab193733 with Assay Diluent A

Adiponectin Mouse ELISA kit - ab108785 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity = 0.7 ng/ml
Range 0.78 ng/ml - 50 ng/ml
Adiponectin measured in biological fluids with background signal subtracted (duplicates +/- SD).

Akt (pS473) + total Akt ELISA Kit - ab126433 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
MCF7 cells were stimulated for five minutes with 1 ug x mL-1 of insulin (ab123768). Cell lysates were assessed for total Akt and Akt(pS473), shown as OD (450 nm) after background signal was subtracted (duplicates +/- SD).

Akt (pS473) ELISA Kit - ab126432 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
Using the CREB1 assay kits CREB1 phosphorylation at Ser133 is detected in EGF-treated A431 cells (+), compared with untreated cells (-), whereas no change in total CREB1 levels was observed.
Jurkat cells were harvested by centrifugation, resuspended in HBSS, and seeded at 10K/well (20 µL/well) in a rinsed PhosphoTracer assay plate. The cells were treated with various concentrations of wortmannin (10 µL/well) for 1 h at 37°C, then treated with 5% FCS (10 µL/well) for 15 min. The cells were lysed with 10 µL/well Lysis Mix Concentrate, with shaking for 10 min. Antibody Mix specific for phospho AKT (50 µL/well) was added to the lysate, and the plates were incubated for 1 hr at room temp, with shaking. Plates were washed and Substrate Mix was added. The plates were covered in foil and incubated for 10 min with shaking. Signal in the wells was determined using a plate reader.

AKT 1/2/3 (pS473) + AKT1 (Total) SimpleStep ELISA™ Kit - ab176657 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 30 pg/ml

AKT 1/2/3 (pS473) PhosphoTracer ELISA Kit - ab119614 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 10 pg/ml
Range 10 pg/ml - 10000 pg/ml
Using the PhosphoTracer AKT 1/2/3 (pS473) assay, the length of assay incubation time that was required to detect recombinant phospho-AKT was examined. After 15 mins, phospho-AKT concentrations of approximately 100 pg/mL were readily detected, while after 1 hour incubation, the limit of detection was less than 10 pg/mL. Longer incubation times had little effect on overall limit of detection that was observed.

AKT 1/2/3 (pS473) SimpleStep ELISA™ Kit - ab176635 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 30 pg/ml
Range 1 ng/ml - 100 ng/ml

AKT 1/2/3 (pT308) + AKT1 (Total) SimpleStep ELISA™ Kit - ab176658 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 30 pg/ml

AKT 1/2/3 (pT308) PhosphoTracer ELISA Kit - ab119615 from Abcam

Category ELISA Kit
Sensitivity 50 pg/ml
Range 100 pg/ml - 10000 pg/ml
Detection of AKT 1/2/3 (pT308) in MCF7 cells treated with various concentration of wortmannin, using ab119615.

AKT 1/2/3 (pT308) SimpleStep ELISA™ Kit - ab176636 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 30 pg/ml
Range 1 ng/ml - 100 ng/ml
Using the AKT 1/2/3 (p-Thr308) assay kits or Western blot, AKT 1/2/3 phosphorylation at Thr308 is detected in serum-treated HEK293 cells (+), compared with cells treated with 2 µM wortmannin (-), whereas no change in total AKT 1/2/3 levels was observed.

AKT total + Phospho S473 In-Cell ELISA Kit (IR) - ab126579 from Abcam

Category ELISA Kit
Prices $448.00
Sizes 1 x 96 tests
Figure 1. Normalized IR signal in PDGF induced NIH3T3 cells. NIH3T3 cells were seeded at 40,000 cells per well and allowed to adhere for 2 hours prior to serum starvation and induction of phosphorylation with a dose-response of PDGF recombinant protein. At maximum doses, PDGF induced a 5 fold increase in the levels of Akt-1 phosphorylation.

AKT total + Phospho S473 In-Cell ELISA kit (Colorimetric) - ab126578 from Abcam

Category ELISA Kit
Prices $448.00
Sizes 1 x 96 tests
Figure 1. Normalized IR signal in PDGF induced NIH3T3 cells. NIH3T3 cells were seeded at 40,000 cells per well and allowed to adhere for 2 hours prior to serum starvation and induction of phosphorylation with a dose-response of PDGF recombinant protein. At maximum doses, PDGF induced a 5 fold increase in the levels of Akt-1 phosphorylation.

Akt Total + pSer473 In-Cell ELISA kit (Fluorescent) - ab156029 from Abcam

Category ELISA Kit
Prices $438.00
Sizes 1 x 96 tests
Range 12000 cells/well - 100000 cells/well
Western blot was run on a 10-20% gradient acrylamide gel. Samples were loaded as follows from left to right: (1) 50ng of Human recombinant AKT1 protein (tagged) Cat# ab62279, (2) 25µg of non-induced NIH3T3 cell extract and (3) 25µg of PDGF induced NIH3T3 cell extract. Membrane Blocking was carried out with 5% Milk+50mM Tris+0.05% Tween-20 pH 7.4, primary antibodies (A) Total AKT1 and (B) pSer473, were incubated overnight in 5% BSA+50mM+0.05% Tween-20 pH 7.4 and secondary antibodies were incubated for 2 hours in 5% Milk+50mM Tris+0.05% Tween-20 pH 7.4.

AKT1 Total PhosphoTracer ELISA Kit - ab119616 from Abcam

Category ELISA Kit
Sensitivity 100 pg/ml
Range 100 pg/ml - 100000 pg/ml
Detection of total AKT1 in MCF7 cells treated with various concentration of wortmannin, using ab119616.

AKT1 Total SimpleStep ELISA™ Kit - ab176637 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 60 pg/ml
Range 0.5 ng/ml - 50 ng/ml

Albumin Mouse ELISA Kit - ab108792 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity = 1.5 ng/ml
Range 1.56 ng/ml - 400 ng/ml
Representative Standard Curve Using ab108792.

Albumin Mouse ELISA Kit - ab108791 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity = 0.09 µg/ml
Range 0.098 µg/ml - 25 µg/ml
Albumin measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
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