Cell differentiation



Active human Activin A full length protein - ab50051 from Abcam

Category Protein
Prices $110.00, $257.00, $316.00
Sizes 2 µg, 5 µg, 10 µg
Species Human
Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Activin A (ab50051) and Wnt3a ( ab81484 ) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 ( ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

Active human FGF basic full length protein - ab9596 from Abcam

Category Protein
Prices $110.00, $257.00
Sizes 10 µg, 50 µg
Source E. coli
Species Human
Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2007. BMP-4, FGF-basic (ab9596) and VEGF ( ab9571 ) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 ( ab32457 in green) and CD34 ( ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.

Active human VEGF full length protein - ab9571 from Abcam

Category Protein
Prices $110.00, $257.00
Sizes 2 µg, 10 µg
Source E. coli
Species Human
Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2006. BMP-4, FGF-basic ( ab9596 ) and VEGF (ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 ( ab32457 in green) and CD34 ( ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.

Active KGF full length protein - ab9601 from Abcam

Category Protein
Prices $110.00, $257.00
Sizes 2 µg, 10 µg
Source E. coli
Definitive endoderm derived from culturing human embryonic stem cells (HUES4) with Inhibin beta a ( ab50051 ) and Wnt3a ( ab81484 ) was further differentiated to gut tube endoderm as described in Kroon et al, Nat Biotechnol. (2008). KGF (ab9601) was added to the media to direct differentiation towards gut tube endoderm. This image was taken 3 days after addition of KGF, where the gut tube endoderm marker TCF2 ( ab59118 in green) is present. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

Active mouse Wnt3a full length protein - ab81484 from Abcam

Category Protein
Prices $300.00
Sizes 10 µg
Source Cell Culture
Species Mouse
Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A ( ab50051 ) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 ( ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

Human FOXA3 full length protein - ab134839 from Abcam

Category Protein
Source E. coli
Species Human

Human FOXD3 full length protein - ab134848 from Abcam

Category Protein
Source E. coli
Species Human

Human Hex full length protein - ab134846 from Abcam

Category Protein
Source E. coli
Species Human

Human VTN-GBF8 protein fragment - ab182834 from Abcam

Category Protein
Source E. coli
Species Human
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