Active mouse Wnt3a full length protein
| Name | Active mouse Wnt3a full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab81484 |
| Category | Protein |
| Prices | $300.00 |
| Sizes | 10 µg |
| Applications | Cell differentiation SDS-PAGE FA |
| Species Reactivities | Mouse |
| Nature | Recombinant |
| Source | Cell Culture |
| Purity | 70 - 90% by HPLC. Greater than 75% by SDS-PAGE gel and HPLC analyses. |
| Bioactivity | The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50for this effect is 0.4-0.6 ng/ml. |
| Endotoxin | < 0.100 Eu/µg |
| SwissProt/Accession | P56704 |
| Gene | WNT3A |
| Sequence | SLAVGPQYSS LSTQPILCAS IPGLVPKQLR FCRNYVEIMP SVAEGVKAGI QECQHQFRGR RWNCTTVSNS LAIFGPVLDK ATRESAFVHA IASAGVAFAV TRSCAEGSAA ICGCSSRLQG SPGEGWKWGG CSEDIEFGGM VSREFADARE NRPDARSAMN RHNNEAGRQA IASHMHLKCK CHGLSGSCEV KTCWWSQPDF RTIGDFLKDK YDSASEMVVE KHRESRGWVE TLRPRYTYFK VPTERDLVYY EASPNFCEPN PETGSFGTRD RTCNVSSHGI DGCDLLCCGR GHNARTERRR EKCHCVFHWC CYVSCQECTR VYDVHTCK |
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Product images
Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A ( ab50051 ) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 ( ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.