Purification



10kD Spin Column - ab93349 from Abcam

Category Cellular Assay
Prices $258.00
Sizes 25 units

Cell Fractionation Kit - Standard - ab109719 from Abcam

Category Cellular Assay
Prices $206.00
Sizes 1 kit
Cytosolic (C), mitochondrial (M) and nuclear (N) fractions of HepG2 cells were prepared as described in the Protocol. Fractions were analyzed by Western blotting using Abcam's ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415) containing antibodies against mitochondrial matrix (pyruvate dehydrogenase subunit E1α, PDH E1α), mitochondrial inner membrane (F1-ATPase α), mitochondrial intermembrane space (cytochrome c) and cytosolic (glyceraldehyde-3-phosphate dehydrogenase, GAPDH) markers as well as with antibodies against additional mitochondrial matrix (Hsp70) and nuclear (poly (ADP-ribose) polymerase, PARP and SP1) markers, followed by appropriate HRP-conjugated goat secondary antibodies and ECL detection.

Circulating DNA Quantification Kit - ab156898 from Abcam

Category Cellular Assay
Prices $175.00, $314.00
Sizes 48 tests, 96 tests
Sensitivity >= 10 ng/ml
Range 10 ng/ml - 1000 ng/ml
Typical standard curve generated using the Circulating DNA Quanitfication Kit (ab156898).

DNA Isolation Kit - Plasma/Serum - ab156893 from Abcam

Category Cellular Assay
Prices $129.00, $227.00
Sizes 50 tests, 100 tests
Range 0.1 ng - 2000 ng
After treatment with DNA digestion buffer, the DNA is easily recovered in 8-20 µl by our specially designed F-Spin Column. DNA is ready for down-stream application.

DNA Isolation Kit - Urine - ab156899 from Abcam

Category Cellular Assay
Prices $129.00, $252.00
Sizes 50 tests, 100 tests
Range 1 ng - 2000 ng
Genomic DNA was isolated from Human urine using ab156899. The isolated DNA yield was quantified by real time PCR.

Gel DNA Purification Kit - ab156896 from Abcam

Category Cellular Assay
Prices $93.00, $149.00
Sizes 50 tests, 100 tests
Sensitivity > 1 ng
Range 1 ng - 1000 ng

Genomic DNA Extraction Kit - ab156900 from Abcam

Category Cellular Assay
Prices $139.00, $252.00
Sizes 50 tests, 100 tests
Range 1 ng - 4000 ng
Genomic DNA was isolated from MCF7 cell line using ab156900. The isolated DNA yield was quantified by real time PCR.

Genomic DNA Isolation Kit - ab65358 from Abcam

Category Cellular Assay
Prices $335.00
Sizes 50 tests

Lipolysis Isolation and Quantification Assay Kit (Colorimetric/Fluorometric) - ab196987 from Abcam

Category Cellular Assay
Prices $525.00
Sizes 5 g
Measurement of glycerol level from adipocyte samples (20 µL of 1/10 diluted sample) using fluorometric assay. Adipocytes were either treated with vehicle control (H2O) or 100 nM isoproterenol for 0-3 hours.

Malate Dehydrogenase 2 (MDH2) Activity Assay - ab119693 from Abcam

Category Cellular Assay
Prices $448.00
Sizes 1 x 96 tests
Sensitivity 0.78 µg/ml
Range 0.78 µg/ml - 200 µg/ml
Figure 1. MDH2 activity measurements of serially diluted cultured HepG2 cell extracts.

Plasma Membrane Protein Extraction Kit - ab65400 from Abcam

Category Cellular Assay
Prices $525.00
Sizes 50 tests

Triglyceride Standard - ab103967 from Abcam

Category Cellular Assay
Prices $145.00
Sizes 300 µl
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