Cell Fractionation Kit - Standard
| Name | Cell Fractionation Kit - Standard |
|---|---|
| Supplier | Abcam |
| Catalog | ab109719 |
| Category | Cellular Assay |
| Prices | $206.00 |
| Sizes | 1 kit |
| Assay Type | Direct |
| Applications | Purification |
| Species Reactivities | Human, Broad species |
| Supplier Page | Shop |
Product images
Cytosolic (C), mitochondrial (M) and nuclear (N) fractions of HepG2 cells were prepared as described in the Protocol. Fractions were analyzed by Western blotting using Abcam's ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415) containing antibodies against mitochondrial matrix (pyruvate dehydrogenase subunit E1α, PDH E1α), mitochondrial inner membrane (F1-ATPase α), mitochondrial intermembrane space (cytochrome c) and cytosolic (glyceraldehyde-3-phosphate dehydrogenase, GAPDH) markers as well as with antibodies against additional mitochondrial matrix (Hsp70) and nuclear (poly (ADP-ribose) polymerase, PARP and SP1) markers, followed by appropriate HRP-conjugated goat secondary antibodies and ECL detection.
In this experiment, apoptosis was induced in Jurkat, HeLa and 143B osteosarcoma cells by treatment with staurosporine or in Jurkat cells by FAS. Mitochondrial and cytoplasmic fractions were isolated (using ab109719) and probed using ab110415. As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.
Product References
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Kalifa L, Gewandter JS, Staversky RJ, Sia EA, Brookes PS, O'Reilly MA. Free Radic Biol Med. 2014 Oct;75:30-9.
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Ameri K, Rajah AM, Nguyen V, Sanders TA, Jahangiri A, Delay M, Donne M, Choi HJ, Tormos KV, Yeghiazarians Y, Jeffrey SS, Rinaudo PF, Rowitch DH, Aghi M, Maltepe E. PLoS One. 2013 Apr 30;8(4):e62758.
MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that - MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that
Hatle KM, Gummadidala P, Navasa N, Bernardo E, Dodge J, Silverstrim B, Fortner K, Burg E, Suratt BT, Hammer J, Radermacher M, Taatjes DJ, Thornton T, Anguita J, Rincon M. Mol Cell Biol. 2013 Jun;33(11):2302-14.
Inhibition of mitochondrial fission prevents cell cycle progression in lung - Inhibition of mitochondrial fission prevents cell cycle progression in lung
Rehman J, Zhang HJ, Toth PT, Zhang Y, Marsboom G, Hong Z, Salgia R, Husain AN, Wietholt C, Archer SL. FASEB J. 2012 May;26(5):2175-86.
Leukotoxin (Leukothera(R)) targets active leukocyte function antigen-1 (LFA-1) - Leukotoxin (Leukothera(R)) targets active leukocyte function antigen-1 (LFA-1)
DiFranco KM, Gupta A, Galusha LE, Perez J, Nguyen TV, Fineza CD, Kachlany SC. J Biol Chem. 2012 May 18;287(21):17618-27.
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