IHC-F | Bioprodhub, a Bio-Reagent Search Engine

IHC-F Immunohistochemistry (Frozen)

5 Hydroxy Tryptophan (conjugated) antibody - ab8890 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anthranilic acid (conjugated) antibody - ab36926 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-14-3-3 (phospho S58) antibody - ab30554 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilutionLane 1 : Rat brain stem lysateLane 2 : Rat brain stem lysate with the phosphopeptide used as the antigenObserved band size : 29 kDa (why is the actual band size different from the predicted?)

Anti-14-3-3 epsilon antibody [EPR3918] - ab92311 from Abcam

Prices	$391.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR3918

Overlay histogram showing SH-SY5Y cells stained with ab92311 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92311, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-140 kD + 160 kD Neurofilament antibody [RNF403] - ab134278 from Abcam

Prices	$407.00
Sizes	50 µl
Host	Mouse
Clonality	Monoclonal
Clone	RNF403

Immunohistochemical analysis of paraffin-embedded Human brain (cortex) tissue labelling 140 kD + 160 kD Neurofilament with ab134278 at 10 &microg/ml. Primary incubation was followed by biotinylated horse anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-145 kD Neurofilament antibody [2E30] - ab35953 from Abcam

Prices	$359.00
Sizes	150 µl
Host	Mouse
Clonality	Monoclonal
Clone	2E30

Anti-15 Lipoxygenase 2 antibody - ab23691 from Abcam

Prices	$394.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-15 Lipoxygenase 2 antibody (ab23691) at 4 µg/mlLane 1 : Rabbit reticulocyte lysate (30 µg).Lane 2 : Human prostate homogenate (30 µg).

Anti-160 kD Neurofilament Medium antibody - ab64300 from Abcam

Prices	$394.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-160 kD Neurofilament Medium antibody (ab64300) at 1 µg/ml + Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Anti-160 kD Neurofilament Medium antibody - ab39371 from Abcam

Prices	$378.00
Sizes	100 µl
Host	Chicken
Clonality	Polyclonal

Ab39371 staining Neurofilament in neonatal rat cortical neurons cultured for 14-21 days.

Anti-160 kD Neurofilament Medium antibody - ab134458 from Abcam

Prices	$378.00
Sizes	200 µl
Host	Chicken
Clonality	Polyclonal

Immunofluorescent analysis of dissociated culture of adult rat brain labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining.

Anti-160 kD Neurofilament Medium antibody [3H11] - ab7256 from Abcam

Prices	$379.00
Sizes	50 µl
Host	Mouse
Clonality	Monoclonal
Clone	3H11

Lane 1 : Coomassie Blue stainLane 2 : Heavy neurofilament antibodyLane 3 : Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)Lane 4 : Anti-68kDa Neurofilament antibody [DA2] (ab7255)Lane 5 : alpha Internexin antibodyLane 6 : GFAP antibodyLane 1 : Cytoskeletal homogenates of rat spinal cordLane 2 : Cytoskeletal homogenates of rat spinal cordLane 3 : Cytoskeletal homogenates of rat spinal cordLane 4 : Cytoskeletal homogenates of rat spinal cordLane 5 : Cytoskeletal homogenates of rat spinal cordLane 6 : Cytoskeletal homogenates of rat spinal cord

Anti-160 kD Neurofilament Medium antibody [3H11] - ab7256 from Abcam

Prices	$379.00
Sizes	50 µl
Host	Mouse
Clonality	Monoclonal
Clone	3H11

Anti-160 kD Neurofilament Medium antibody [NF-09] - ab7794 from Abcam

Prices	$390.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	NF-09

ab7794 at a dilution of 1/1000, staining 160 kD Neurofilament Medium (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

Anti-160 kD Neurofilament Medium antibody [RMO44] - ab50284 from Abcam

Prices	$388.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	RMO44

ab50284 staining 160 kD Neurofilament Medium in 16 µm thick sections of Apteronotus leptoryhnchus brain tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS and then incubated with ab50284 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488-conjugated goat anti-mouse polyclonal used at a 1/200 dilution.See Abreview

Anti-160 kD Neurofilament Medium antibody - Neuronal Marker - ab9034 from Abcam

Prices	$390.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

The image illustrates the high definition dendritic staining obtained after protease pretreatment. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).

Anti-160 kD Neurofilament Medium antibody - Neuronal Marker - ab9034 from Abcam

Prices	$390.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-200 kD Neurofilament Heavy (phospho ) antibody [SMI-36] - ab24572 from Abcam

Host	Mouse
Clonality	Monoclonal
Clone	SMI-36

Anti-200 kD Neurofilament Heavy (phospho ) antibody [SMI-36] - ab24572 from Abcam

Host	Mouse
Clonality	Monoclonal
Clone	SMI-36

Anti-200 kD Neurofilament Heavy antibody - ab4680 from Abcam

Prices	$401.00
Sizes	25 µl
Host	Chicken
Clonality	Polyclonal

Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).

Anti-200 kD Neurofilament Heavy antibody - ab4680 from Abcam

Prices	$401.00
Sizes	25 µl
Host	Chicken
Clonality	Polyclonal

Anti-200 kD Neurofilament Heavy antibody - ab88043 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ab88043 staining 200kDa Neurofilament Heavy in Zebrafish retina (neurons) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Tris-HCl was used for antigen retrieval. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview

Anti-200 kD Neurofilament Heavy antibody - ab105453 from Abcam

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC image of 200kD Neurofilament Heavy staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105453, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-200 kD Neurofilament Heavy antibody - ab134459 from Abcam

Prices	$378.00
Sizes	200 µl
Host	Chicken
Clonality	Polyclonal

Immunofluorescent analysis of dissociated cell culture prepared from an adult rat brain labelling 200kD Neurofilament Heavy with ab134459 at 1/2000 dilution. 200kD Neurofilament Heavy (green staining) can be found within the neuronal cell body and in the neurites.

Anti-200 kD Neurofilament Heavy antibody - ab92617 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Chicken
Clonality	Polyclonal

ab92617, at 1/1000 dilution, staining a 200 kD Neurofilament Heavy-positive cultured neuron (green) from a neonatal mouse brain by Immunofluorescence.

Anti-200 kD Neurofilament Heavy antibody [AH1] - ab78158 from Abcam

Host	Mouse
Clonality	Monoclonal
Clone	AH1

Anti-200 kD Neurofilament Heavy antibody [AH1] (ab78158) at 1/10000 dilution + Crude rat spinal cord extractdeveloped using the ECL technique

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