APH1A

Gene Symbol APH1A
Entrez Gene 51107
Alt Symbol 6530402N02Rik, APH-1, APH-1A, CGI-78
Species Human
Gene Type protein-coding
Description APH1A gamma secretase subunit
Other Description anterior pharynx defective 1 homolog A|aph-1alpha|gamma-secretase subunit APH-1A|presenilin-stabilization factor
Swissprots Q9Y386 Q9BVG0 B4DQK0 Q96BI3 Q5TB23 Q969R6 Q5TB22
Accessions CAF86638 CAI22811 CAI22812 CAI22813 EAW53565 EAW53566 EAW53567 Q96BI3 AF151835 AAD34072 AF508787 AAN63816 AK027879 BAG51389 AK075295 BAC11529 AK075452 BAG52142 AK125685 AK297680 BAG60040 AK298832 BAG60962 AK298876 BAG60993 AK300640 BAG62329 AK307358 AK314490 AX775955 CAE11677 AX775957 CAE11678 AY113698 AAM61955 AY113699 AAM61956 AY358951 AAQ89310 BC001230 AAH01230 BC008732 AAH08732 BC009501 AAH09501 BC015568 AAH15568 BC017699 AAH17699 BC020590 AAH20590 BC068606 BI760743 DA589586 DC365601 HQ447145 ADQ31632 JF432669 ADZ15886 JF432870 ADZ16087 XM_006711362 XP_006711425 NM_001077628 NP_001071096 NM_001243771 NP_001230700 NM_001243772 NP_001230701 NM_016022 NP_057106 NR_045033 NR_045034 NR_045035
Function Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. {ECO:0000269|PubMed:12297508, ECO:0000269|PubMed:12522139, ECO:0000269|PubMed:12763021}.
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi- pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Tissue Specificity Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level. {ECO:0000269|PubMed:12110170}.
Top Pathways Notch signaling pathway, Alzheimer's disease

Aph-1 (D-20) - sc-30242 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Aph-1 (D-20): sc-30242. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells.

Aph-1 (H-50) - sc-98469 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Aph-1 (N-20) - sc-30240 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Aph-1 (N-20): sc-30240. Western blot analysis of Aph-1 expression in HeLa whole cell lysate.

Anti-APH1a antibody - ab12104 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-APH1a antibody (ab12104) at 1/500 dilutionLane 1 : 20ug human brain lysateLane 2 : 20ug mouse brain lysateLane 3 : 20ug human brain lysate with Human APH1a peptide (ab12394) at 1 µg/mlLane 4 : 20ug mouse brain lysate with Human APH1a peptide (ab12394) at 1 µg/mlSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilutionPerformed under reducing conditions.

Anti-APH1a antibody - ab62821 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-APH1a antibody (ab62821) at 0.05 µg/ml + Brain (Cerebral Cortex) lysate (in RIPA buffer) at 35 µg

Anti-APH1a antibody - Aminoterminal end - ab62521 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 0.5 µg/mlLane 2 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 1 µg/mlLane 3 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 2 µg/mlLane 1 : RAW264.7 cell lysate Lane 2 : RAW264.7 cell lysate Lane 3 : RAW264.7 cell lysate Lysates/proteins at 15 µg per lane.

Anti-APH1a antibody - ChIP Grade - ab19390 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-APH1a antibody - N-terminal - ab60119 from Abcam

Prices $304.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-APH1a antibody - N-terminal (ab60119) at 1/100 dilution

Anti-APH1A / APH-1 Antibody (C-Terminus) - LS-C51634 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Western blot analysis: Lane 1: Mouse liver tissue lysate Lane 2: HeLa cell lysate Primary antibody: 2 ug/ml Rabbit Anti-APH1 Polyclonal Antibody LS-C51634 Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody The signal was developed with LumiSensor HRP Substrate Kit

Anti-APH1A / APH-1 Antibody (aa140-158) - LS-C149030 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (aa41-70, AP) - LS-C232746 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (aa41-70, APC) - LS-C232747 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (aa41-70, Biotin) - LS-C232748 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (aa41-70, FITC) - LS-C232749 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (N-Terminus, AP) - LS-C243679 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (aa41-70, PE) - LS-C232750 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (aa80-130) - LS-C148727 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of APH1A in 1) human lung lysate using pre bleed at 1:5000, 2) human lung lysate using LS-C148727 at 5 ug/ml and 3) rat lung lysate using LS-C148727 at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test.

Anti-APH1A / APH-1 Antibody (N-Terminus, APC) - LS-C243680 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (N-Terminus, Biotin) - LS-C243681 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (N-Terminus, PE) - LS-C243684 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (N-Terminus, FITC) - LS-C243682 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (N-Terminus, Azide-free, HRP) - LS-C247439 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (aa41-70, Azide-free, HRP) - LS-C249440 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (N-Terminus) - LS-C326852 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-APH1A / APH-1 Antibody (aa77-109) - LS-C100036 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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