RAD51

Gene Symbol RAD51
Entrez Gene 396086
Alt Symbol RAD51A
Species Chicken
Gene Type protein-coding
Description RAD51 recombinase
Other Description DNA repair protein RAD51 homolog 1|RAD51 homolog (RecA homolog, E. coli)|homolog to S.cerevisiae
Swissprots P37383
Accessions P37383 L09655 S59426 AAB26354 NM_205173 NP_990504
Function Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA (By similarity). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000305}.
Tissue Specificity Expressed at high levels in lymphoid and reproductive organs.
Top Pathways Homologous recombination, Fanconi anemia pathway

Anti-Rad51 (phospho T309) antibody - ab31769 from Abcam

Prices $242.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Elisa using ab31769 at varying antibody concentrations. Curve_SPL3 indicates binding to the Rad51 (phospho T309) peptide. Curve_SPL4 indicates <2% crossreactivity with the unmodified Rad51 control peptide.

Anti-Rad51 antibody - ab63801 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad51 antibody (ab63801) at 1/2000 dilutionLane 1 : Molecular weight markersLane 2 : HeLa cell extract

Anti-Rad51 antibody - ab137323 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Cal27 Human xenograft tissue labelling Rad51, using ab137323 at a 1/100 dilution.

Anti-Rad51 antibody - ab84304 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab84304 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84304, 5ug/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Rad51 antibody - ChIP Grade - ab176458 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Rad51 antibody - ChIP Grade (ab176458) at 1/1000 dilution + Crude HeLa cell extracts at 10 µgSecondaryGoat anti-Rabbit IgG conjugated to HRP at 1/20000 dilution

Anti-Rad51 antibody - ChIP Grade - ab179897 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad51 antibody - ChIP Grade (ab179897) at 1/1000 dilutionLane 1 : HeLa crude extract from cells grown in the absence of adriamycin (DNA damaging agent)Lane 2 : HeLa crude extract from cells grown in the presence of adriamycin (DNA damaging agent)Lane 3 : NIH 3T3 crude extract
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