MNAT1

Gene Symbol MNAT1
Entrez Gene 4331
Alt Symbol CAP35, MAT1, RNF66, TFB3
Species Human
Gene Type protein-coding
Description MNAT CDK-activating kinase assembly factor 1
Other Description CDK-activating kinase assembly factor MAT1|CDK7/cyclin-H assembly factor|RING finger protein 66|RING finger protein MAT1|cyclin G1 interacting protein|cyclin-G1-interacting protein|menage a trois 1 (CAK assembly factor)|menage a trois homolog 1, cyclin H assembly factor
Swissprots Q15817 G3V1U8 Q6ICQ7 P51948
Accessions AAN47195 EAW80787 EAW80788 EAW80789 EAW80790 EAW80791 P51948 AA053721 AK308701 BC000820 AAH00820 BU620200 CD701743 CR450336 CAG29332 HQ447795 ADQ32279 U61835 AAB05248 X87843 CAA61112 X92669 CAA63356 XM_005267687 XP_005267744 XM_005267688 XP_005267745 XM_011536795 XP_011535097 NM_001177963 NP_001171434 NM_002431 NP_002422
Function Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. {ECO:0000269|PubMed:10024882}.
Subcellular Location Nucleus.
Tissue Specificity Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
Top Pathways Nucleotide excision repair, Basal transcription factors

Mat1 (F-6) - sc-13142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Mat1 (F-6): sc-13142. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Mat1 (C-167) - sc-6235 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Mat1 (C-167): sc-6235. Western blot analysis of Mat1 expression in A-431 (A) and NIH/3T3 (B) whole cell lysates and His-tagged human recombinant Mat1 (C).

Mat1 (K-17) - sc-1601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Mat1 (FL-309) - sc-6234 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Mat1 (FL-309): sc-6234. Western blot analysis of Mat1 expression in A-431 (A) and A-673 (B) nuclear extracts.

Mat1 (M-20) - sc-1600 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Mat1 (19) - sc-136540 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Mat1 (19): sc-136540. Western blot analysis of Mat1 expression in K-562 whole cell lysate.

Mat1 (6) - sc-135981 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Mat1 (6): sc-135981. Western blot analysis of Mat1 expression in mouse macrophage tissue extract.

Anti-MNAT1 antibody - ab175398 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling MNAT1 with ab175398 at 1/200. Magnification: 200x.

Anti-MNAT1 antibody - ab199673 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MNAT1 antibody (ab199673) at 1/1000 dilution + human testis tissue lysate at 35 µgSecondaryGoat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

Anti-MNAT1 antibody - ab65125 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MNAT1 antibody (ab65125) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HepG2 cell extractLane 3 : 293 cell extractLane 4 : 293 cell extract with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-MNAT1 antibody [EPR10940] - ab169546 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10940
All lanes : Anti-MNAT1 antibody [EPR10940] (ab169546) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Anti-MNAT1 antibody [EPR10940-92] - ab171965 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10940-92
All lanes : Anti-MNAT1 antibody [EPR10940-92] (ab171965) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : rat testis lysateLane 3 : mouse testis lysateLane 4 : HepG2 cell lysateLane 5 : HeLa cell lysateLane 6 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-MNAT1 antibody [EPR2554(2)] - ab169774 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2554(2)
Flow Cytometrical analysis of permeabilized HeLa cells with ab169774 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

Anti-MNAT1 antibody [EPR2555(2)] - ab154802 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2555(2)
All lanes : Anti-MNAT1 antibody [EPR2555(2)] (ab154802) at 1/1000 dilutionLane 1 : A431 lysateLane 2 : HeLa lysateLane 3 : A673 lysateLane 4 : RAW264.7 lysateLysates/proteins at 10 µg per lane.

Anti-MNAT1 Antibody - LS-C335190 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded rat brain using MNAT1 antibody at dilution of 1:200 (200x lens).

Anti-MNAT1 Antibody (C-Terminus) - LS-C30746 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Jurkat Cell Lysate

Anti-MNAT1 Antibody (N-Terminus) - LS-C82063 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-MNAT1 Antibody (aa91-140) - LS-C118925 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa/HepG2/293 cells, using MAT1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-MNAT1 Antibody - LS-C192838 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of various cell lines, using MNAT1 antibody.

MNAT1 Antibody - NBP1-81257 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: MNAT1 Antibody [NBP1-81257] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

MNAT1 Polyclonal Antibody - bs-7896R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MNAT1 antibody produced in rabbit - AV38150 from Sigma-Aldrich

Prices $242.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: Jurkat (<B>Cat. No. AV38150</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1.25 μg/mL in cell type Jurkat

Anti-MAT1 antibody produced in rabbit - SAB4501909 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MAT1: <B>Cat. No. SAB4501909</B>: Immunoblotting analysis of extracts from HeLa/HepG2/293 cells, using Anti-MAT1 antibody. The lane on the left was treated with the Anti-MAT1 antibody. The lane on the right (negative control) was treated with both Anti-MAT1 antibody and the synthesized immunogen peptide.

Anti-MNAT1 antibody produced in rabbit - AV100992 from Sigma-Aldrich

Prices $328.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: 721_B (<B>Cat. No. AV100992</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1 μg/mL in cell type 721_B

Anti-MNAT1 antibody produced in rabbit - HPA000701 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MNAT1: <B>Cat. No. HPA000701</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in ductus seminiferus.
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