UGCG

Gene Symbol	UGCG
Entrez Gene	7357
Alt Symbol	GCS, GLCT1
Species	Human
Gene Type	protein-coding
Description	UDP-glucose ceramide glucosyltransferase
Other Description	UDP-glucose:N-acylsphingosine D-glucosyltransferase\|ceramide glucosyltransferase\|glucosylceramide synthase
Swissprots	Q5T258 Q16739
Accessions	EAW59091 EAW59092 Q16739 AJ420423 AK314847 BAG37364 BC038711 AAH38711 BM052821 D50840 BAA09451 JF432523 ADZ15740 XM_005252186 XP_005252243 NM_003358 NP_003349
Function	Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase". {ECO:0000269\|PubMed:8643456}.
Subcellular Location	Golgi apparatus membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
Tissue Specificity	Found in all tissues examined. {ECO:0000269\|PubMed:8643456}.
Top Pathways	Sphingolipid metabolism

UGCG (H-300) - sc-50511 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

UGCG (H-300): sc-50511. Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.

UGCG (E-14) - sc-33869 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-Ceramide glucosyltransferase antibody - ab124296 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue Lysate Lane 4 : Spleen (Mouse) Tissue LysateLane 5 : Spleen (Rat) Tissue LysateLane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 7 : A375 (Human melanoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Ceramide glucosyltransferase antibody - ab197369 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Ceramide glucosyltransferase antibody (ab197369) at 1/800 dilution + A431 cell lysate at 40 µgdeveloped using the ECL technique

Anti-Ceramide glucosyltransferase antibody - ab98030 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Ceramide glucosyltransferase antibody (ab98030) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-UGCG Antibody (aa39-171, FITC) - LS-C303490 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-UGCG Antibody (aa39-171, Biotin) - LS-C299828 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-UGCG Antibody - LS-C314069 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-UGCG Antibody (aa35-84) - LS-C107639 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

UGCG antibody LS-C107639 Western blot of Fetal Spleen lysate.

Anti-UGCG Antibody (clone 1E5) - LS-C198403 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1E5

Detection limit for recombinant GST tagged UGCG is 0.3 ng/ml as a capture antibody.

Anti-UGCG Antibody - LS-C26836 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Ceramide Glycosyltransferase antibody (LS-C26836) on 7 ug of rat kidney lysate. Antibody used at 1 ug/ml.

Anti-UGCG Antibody (aa39-171) - LS-C296910 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-GCLM Antibody (Val91) - LS-C177280 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of GCLM (V91) pAb in paraffin-embedded human prostate carcinoma tissue.

Anti-UGCG Antibody - LS-C334637 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of Mouse brain cell line, using UGCG antibody.

UGCG Antibody (1E5.) - H00007357-M03 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1E5.

Western Blot: UGCG Antibody (1E5) [H00007357-M03] - Analysis of UGCG expression in HL-60.

UGCG Antibody - NBP1-62512 from Novus Biologicals

Prices	$369.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: UGCG Antibody [NBP1-62512] - Titration: 0.2-1 ug/ml, Positive Control: Human Spleen.

UGCG Antibody - NBP1-83376 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: UGCG Antibody [NBP1-83376] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

UGCG Antibody - H00007357-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: UGCG Antibody [H00007357-A01]

Ceramide glucosyltransferase Antibody - bs-0701R from Bioss

Prices	$279.50
Sizes	100 µl
Host	Rabbit

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti GCS/glucosylceramide synthase Polyclonal Antibody, Unconjugated (bs-0701R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

UGCG Polyclonal Antibody - bs-8593R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-UGCG antibody produced in rabbit - HPA024124 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-UGCG: <B>Cat. No. HPA024124</B>: Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.

Anti-UGCG, (N-terminal) antibody produced in rabbit - SAB2104830 from Sigma-Aldrich

Prices	$347.50
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-UGCG, antibody produced in rabbit: <B>Cat. No. SAB2104830</B>: Immunoblotting analysis of UGCG in fetal spleen tissue lysate with UGCG anitbody at 1 μg/mL.

UGCG antibody - N-terminal region (ARP42123_P050) - ARP42123_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

UGCG Antibody (OABF00261) - OABF00261 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

UGCG antibody - 70R-21150 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

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