ALDOA

Gene Symbol ALDOA
Entrez Gene 226
Alt Symbol ALDA, GSD12, HEL-S-87p
Species Human
Gene Type protein-coding
Description aldolase A, fructose-bisphosphate
Other Description epididymis secretory sperm binding protein Li 87p|fructose-1,6-bisphosphate triosephosphate-lyase|fructose-bisphosphate aldolase A|lung cancer antigen NY-LU-1|muscle-type aldolase
Swissprots Q6FI10 Q96B15 P04075 Q6FH76 Q9UCN2 Q9BWD9 B4DXI7
Accessions CAA29654 CAA30979 EAW79933 EAW79934 EAW79935 EAW79936 P04075 AK026577 AK098778 AK301993 BAG63399 AK308495 AK308748 BC000367 AAH00367 BC004333 AAH04333 BC010660 AAH10660 BC012880 AAH12880 BC013614 AAH13614 BC015888 AAH15888 BC016170 AAH16170 BC016800 AAH16800 BE893518 BQ719865 BU855133 BX647566 CB151262 CR536528 CAG38765 CR541880 CAG46678 D28356 BAA05722 DA138954 DA564075 DA893541 DA899482 DB057572 DQ891065 ABM81991 DQ894246 ABM85172 EF036496 ABO65082 EF036497 ABO65083 FJ474908 ACQ91105 M11560 AAA51690 M21190 AAA51697 X05236 CAA28861 XM_011545767 XP_011544069 XM_011545768 XP_011544070 NM_000034 NP_000025 NM_001127617 NP_001121089 NM_001243177 NP_001230106 NM_184041 NP_908930 NM_184043 NP_908932 NM_001243175
Function Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+). {ECO:0000250}.
Top Pathways Glycolysis / Gluconeogenesis, Pentose phosphate pathway, Biosynthesis of amino acids, Carbon metabolism, Fructose and mannose metabolism

Aldolase A Antibody - 3188 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, using Aldolase A Antibody.

Aldolase A (D73H4) Rabbit mAb - 8060 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D73H4
Western blot analysis of extracts from various cell lines using Aldolase A (D73H4) Rabbit mAb.

Aldolase A (C-10) - sc-390733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Aldolase A (C-10): sc-390733. Western blot analysis of Aldolase A expression in Caki-1 (A) and A-673 (B) whole cell lysates.

Aldolase A (N-15) - sc-12059 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Aldolase A (N-15): sc-12059. Western blot analysis of Aldolase A expression in A-673 whole cell lysates.

Aldolase A (C-16) - sc-12061 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Aldolase A (C-16): sc-12061. Western blot analysis of Aldolase A expression in non-transfected: sc-117752 (A) and human Aldolase A transfected: sc-113098 (B) 293T whole cell lysates.

Aldolase A (H-45) - sc-30082 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Aldolase A (H-45): sc-30082. Western blot analysis of Aldolase A expression in KNRK whole cell lysate.

Aldolase A (1F8) - sc-53933 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Aldolase A (1F8): sc-53933. Western blot analysis of Aldolase A expression in non-transfected 293T: sc-117752 (A), human Aldolase A transfected 293T: sc-170532 (B) and A-673 (C) whole cell lysates.

Anti-Aldolase antibody - ab54770 from Abcam

Prices $397.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Aldolase was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to Aldolase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54770.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 39kDa; Aldolase

Anti-Aldolase antibody - ab6190 from Abcam

Prices $359.00
Sizes 500 µl
Host Goat
Clonality Polyclonal
IHC image of Aldolase staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins at 98°C. The section was incubated with ab6190, 1/200 dilution, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX

Anti-Aldolase antibody - ab78339 from Abcam

Prices $379.00
Sizes 50 µl
Host Goat
Clonality Polyclonal
ab78339, at a concentration of 5 µg/ml, staining Aldolase in formalin fixed paraffin embedded heart tissue by Immunohistochemistry.

Anti-Aldolase antibody - ab181694 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-Aldolase antibody - ab133729 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Aldolase antibody (ab133729) at 1/60 dilution + CEM lysate at 35 µg

Anti-Aldolase antibody - ab54763 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Aldolase antibody - ab135899 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Aldolase antibody (ab135899) at 1/500 dilution + HT29 cell lysate at 30 µg

Anti-Aldolase antibody - ab184126 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Aldolase antibody (ab184126) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : A431 cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 30 µg per lane.

Anti-Aldolase antibody [1F8] - ab67204 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F8

Anti-Aldolase antibody [2E6] - ab129281 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2E6
Anti-Aldolase antibody [2E6] (ab129281) + HepG2 cell lysate

Anti-Aldolase antibody (Biotin) - ab181744 from Abcam

Prices $372.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-Aldolase antibody [EPR9724(B)] - ab169544 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9724(B)
All lanes : Anti-Aldolase antibody [EPR9724(B)] (ab169544) at 1/1000 dilutionLane 1 : A549 lysateLane 2 : Fetal liver lysateLane 3 : HeLa lysate Lane 4 : Fetal brain lysate Lane 5 : Human skeletal muscle lysate Lysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Anti-Aldolase antibody [EPR9724(B)] (HRP) - ab200774 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9724(B)
All lanes : Anti-Aldolase antibody [EPR9724(B)] (HRP) (ab200774) at 1/5000 dilutionLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 2 : Fetal Liver (Human) Normal Tissue)Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : Fetal Brain (Human) Normal Tissue lysateLane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Aldolase antibody [EPR9725] - ab150396 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9725
All lanes : Anti-Aldolase antibody [EPR9725] (ab150396) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : HepG2 cell lysateLane 3 : A549 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Aldolase antibody (HRP) - ab181662 from Abcam

Prices $384.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Aldolase antibody (HRP) (ab181662) at 1/1000 dilution + A293 whole cell lysatedeveloped using the ECL technique

Anti-Aldolase antibody (HRP) - ab34598 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Aldolase antibody (HRP) (ab34598) at 1/1000 dilution + A293 cell lysate

Anti-Aldolase antibody - BSA and Azide free (Biotin) - ab182880 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Aldolase antibody - C-terminal - ab182664 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Aldolase antibody - C-terminal (ab182664) at 1/500 dilutionLane 1 : A549 cell lysateLane 2 : Hela cell lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutiondeveloped using the ECL technique
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