All lanes : Anti-Aldolase antibody [EPR9724(B)] (HRP) (ab200774) at 1/5000 dilutionLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 2 : Fetal Liver (Human) Normal Tissue)Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : Fetal Brain (Human) Normal Tissue lysateLane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.