HK1

Gene Symbol HK1
Entrez Gene 3098
Alt Symbol HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1
Species Human
Gene Type protein-coding
Description hexokinase 1
Other Description HK I|brain form hexokinase|glycolytic enzyme|hexokinase type I|hexokinase-1
Swissprots O43443 Q9NNZ5 O75574 P19367 O43444 E9PCK0 Q5VTC3 Q96HC8 Q9NNZ4
Accessions AAC00172 AAC00173 AAC15862 AAC15863 AAF82319 AAF82320 EAW54319 EAW54320 EAW54321 EAW54322 EAW54323 P19367 AB209526 BAD92763 AF073786 AAC25424 AK091267 BAG52321 AK128226 BAG54651 AK292012 BAF84701 AK294428 BAG57673 AK309904 BC008730 AAH08730 BX406321 DB088955 DQ893281 ABM84207 DQ896762 ABM87761 M75126 AAA52646 U38226 AAB40527 U38227 AAB40528 U38228 AAB40529 X66957 CAA47379 XM_005269735 XP_005269792 XM_005269736 XP_005269793 XM_005269737 XP_005269794 XM_011539732 XP_011538034 XM_011539733 XP_011538035 XM_011539734 XP_011538036 NM_000188 NP_000179 NM_033496 NP_277031 NM_033497 NP_277032 NM_033498 NP_277033 NM_033500 NP_277035
Subcellular Location Mitochondrion outer membrane. Note=Its hydrophobic N-terminal sequence may be involved in membrane binding.
Tissue Specificity Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
Top Pathways Glycolysis / Gluconeogenesis, Central carbon metabolism in cancer, Carbohydrate digestion and absorption, Type II diabetes mellitus, Insulin signaling pathway

Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 3540 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone C35C4
Confocal immunofluorescent analysis of MCF-7 cells using Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor) and MitoTracker ® Red CMXRos (red) showing colocalization with mitochondria. Nuclei have been labeled with p53 (1C12) Mouse mAb (Alexa Fluor ® 488 Conjugate) #2015 (green).

Hexokinase I (C35C4) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 3689 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone C35C4

Hexokinase I Antibody - 2804 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Hexokinase I Antibody.

Hexokinase I (C35C4) Rabbit mAb - 2024 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C35C4
Western blot analysis of extracts from various cell types using Hexokinase I (C35C4) Rabbit mAb.

HXK I (A-7) - sc-271865 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HXK I (A-7): sc-271865. Western blot analysis of HXK I expression in non-transfected: sc-117752 (A) and human HXK I transfected: sc-170521 (B) 293T whole cell lysates.

HXK I (4D7) - sc-80978 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HXK I (4D7): sc-80978. Western blot analysis of HXK I expression in HeLa (A), Hep G2 (B), SK-N-MC (C), ME-180 (D), HEL 92.1.7 (E) and JAR (F) whole cell lysates.

HXK I (C-14) - sc-6518 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HXK I (C-14): sc-6518. Western blot analysis of HXK I expression in rat brain tissue extract.

HXK I (G-1) - sc-46695 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HXK I (G-1): sc-46695. Western blot analysis of HXK I expression in human (A), rat (B) and mouse (C) brain tissue extracts.

HXK I (H-95) - sc-28885 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HXK I (H-95): sc-28885. Western blot analysis of human recombinant HXK I fusion protein.

HXK I (N-19) - sc-6517 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HXK I (N-19): sc-6517. Western blot analysis of HXK I expression in HeLa (A), Hep G2 (B) and SK-N-MC (C) whole cell lysates.

Anti-Hexokinase 1 antibody - ab55144 from Abcam

Prices $390.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Hexokinase antibody (ab55144) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.

Anti-Hexokinase 1 antibody - ab65069 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hexokinase 1 antibody (ab65069) at 1/500 dilutionLane 1 : extracts from HeLa cellsLane 2 : extracts from HeLa cells with immunizing peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-Hexokinase 1 antibody - ab110529 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hexokinase 1 antibody (ab110529) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Hexokinase 1 antibody - ab128542 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Hexokinase 1 antibody (ab128542) at 1/1000 dilution + K562 cell lysate

Anti-Hexokinase 1 antibody - ab150013 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hexokinase 1 antibody (ab150013) at 1 µg/mlLane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Hexokinase 1 antibody [3A10] - ab104835 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3A10
All lanes : Anti-Hexokinase 1 antibody [3A10] (ab104835) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : NIH3T3 cell lysate

Anti-Hexokinase 1 antibody [4D7] - ab105213 from Abcam

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4D7
ab105213 at 5 µg/ml staining Hexokinase 1 in Human brain (cortex) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Hexokinase 1 antibody [7A7] - ab106176 from Abcam

Host Mouse
Clonality Monoclonal
Clone 7A7
All lanes : Anti-Hexokinase 1 antibody [7A7] (ab106176) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : MCF7 cell lysateLane 5 : PC12 cell lysate

Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker - ab150423 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10134(B)
Overlay histogram showing K562 cells stained with ab150423 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150423, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (Alexa Fluor® 488) - ab184818 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10134(B)
Overlay histogram showing HeLa cells stained with ab184818 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184818, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (Alexa Fluor® 647) - ab197864 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10134(B)
ab197864 staining Hexokinase 1 in HCT116 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (HRP) - ab198534 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10134(B)
All lanes : Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (HRP) (ab198534) at 1/5000 dilutionLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Hexokinase 1 antibody [EPR10135(B)] - ab154839 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10135(B)
All lanes : Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : MCF7 cell lysateLane 3 : U-87 MG cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

Anti-Hexokinase 1 antibody - C-terminal - ab135685 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Hexokinase 1 antibody - C-terminal (ab135685) at 1/100 dilution + A375 cell line lysate at 35 µg

Anti-pan-Hexokinase antibody [1H10-B10-H4] - ab150752 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1H10-B10-H4
All lanes : Anti-pan-Hexokinase antibody [1H10-B10-H4] (ab150752) at 1/10000 dilutionLane 1 : HeLa cell lysatesLane 2 : Jurkat cell lysates
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