ab105213 at 5 µg/ml staining Hexokinase 1 in Human brain (cortex) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
![Anti-Hexokinase 1 antibody [4D7] (ab105213) at 1/1000 dilution + HepG2 cell lysate at 30 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/227_Hexokinase-1-Primary-antibodies-ab105213-3.JPG)
Anti-Hexokinase 1 antibody [4D7] (ab105213) at 1/1000 dilution + HepG2 cell lysate at 30 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique
ab105213 staining Hexokinase 1 in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% NGS, 5% BSA in PBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/250 in diluent) for 16 hours at 4°C. A Goat anti-mouse HRP IgG polyclonal (1/250) was used as the secondary antibody.See Abreview