VEGFA

Gene Symbol VEGFA
Entrez Gene 281572
Alt Symbol VEGF, VEGF-A, VPF, eVEGF120, eVEGF164
Species Bovine
Gene Type protein-coding
Description vascular endothelial growth factor A
Other Description vascular endothelial growth factor 164|vascular permeability factor
Swissprots P15691
Accessions AAV34174 DAA16511 P15691 AB450824 BAH86639 AB455252 BAH86640 EU017524 ABU98339 GU362062 ADV02765 GU362063 ADV02766 M31836 AAA30804 M32976 AAA30502 M33750 AAA30805 NM_174216 NP_776641
Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). {ECO:0000250}.
Subcellular Location Secreted {ECO:0000250}. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin. {ECO:0000250}.
Top Pathways Proteoglycans in cancer, MicroRNAs in cancer, Rap1 signaling pathway, Cytokine-cytokine receptor interaction, PI3K-Akt signaling pathway

Anti-VEGF antibody - ab14078 from Abcam

Host Chicken
Clonality Polyclonal
Immunohistochemical analysis of mouse RPE/Choroidal whole-mounts labeling VEGF with ab14078 at 1:100 dilution. Left panel: purple staining VEGF. Right panel: overlay with Iba1 antibody. VEGF expression is shown in accumulating macrophages following laser induced choroidal neovascularisation.    Eyes were enucleated and fixed in 2% (wt/vol) paraformaldehyde (PFA). After dissection of RPE/choroid, tissues were blocked and permeabilized in 5% BSA, with 0.3% Triton X-100 in PBS for 2 hours, followed by incubation with antibodies in 1% BSA with 0.15% Triton X-100 at 4°C overnight. After thorough washing, samples were further incubated with secondary antibodies at RT for 3 hours in the dark. Tissues were washed and flat-mounted in medium.

Anti-VEGFA antibody - ab136392 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

VEGF-A antibody - AP02022SU-N from Acris Antibodies

Prices $640.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of VEGF staining of Paraffin Sections of Bovine follicules using AP02022SU VEGF antibody. Antigen retrieval was performed by heating the dewaxed sections 4x to 95°C for 5 min. The section was then treated with Hydrogen Peroxide (1%) in methanol for 30 min to block endogenous peroxidase. The section was incubated with AP02022SU (1/300) overnight, followed by staining with ABC method. DAB in 0.0006% Hydogen Peroxide/0.05M Tris buffer (pH7.6) was used as the chromogen. This antibody stains the cytoplasm of granulosa and theca cells of mature follicules.  Scale bar: 100 µm.  Berisha B et al. (2002) J. Endocrinol. 167(3): 371-382.

VEGF-A antibody - AP02022SU-S from Acris Antibodies

Prices $205.00
Sizes 20 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of VEGF staining of Paraffin Sections of Bovine follicules using AP02022SU VEGF antibody. Antigen retrieval was performed by heating the dewaxed sections 4x to 95°C for 5 min. The section was then treated with Hydrogen Peroxide (1%) in methanol for 30 min to block endogenous peroxidase. The section was incubated with AP02022SU (1/300) overnight, followed by staining with ABC method. DAB in 0.0006% Hydogen Peroxide/0.05M Tris buffer (pH7.6) was used as the chromogen. This antibody stains the cytoplasm of granulosa and theca cells of mature follicules.  Scale bar: 100 µm.  Berisha B et al. (2002) J. Endocrinol. 167(3): 371-382.

Anti-VEGFA polyclonal antibody - DPAB-DC1408 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Rabbit anti-Cow VEGFA polyclonal antibody - DPABH-00177 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Rabbit anti-Cow VEGFA polyclonal antibody - DPABH-00182 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

VEGF Polyclonal Antibody - PA1-16948 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of VEGFA doublet in CSF-IR/VEGFA chimera transfected lysate using PA1-16948. ECL 10 second exposure.

VEGF Monoclonal Antibody (VG76e) - MA1-16626 from Thermo Fisher Scientific

Prices $354.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone VG76e

Vascular Endothelial Growth Factor 164, Polyclonal Antibody - MBS619304 from MyBioSource

Prices $435.00, $940.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

VEGF-A Antibody - 200-401-B74 from Rockland Immunochemicals Inc

Prices $319.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot using Rockland's Protein-A Purified anti-bovine VEGF-A antibody shows detection of recombinant bovine VEGF-A at 17-19.2 kDa (arrowhead).   Approximately 2 µg of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane.   The membrane was blocked using 3% BSA (p/n BSA-30) diluted 1:10.  The primary antibody was used at a 1:333 dilution and was incubated with the blot for 2h at room temperature.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10.  Molecular weight estimation was made by comparison to prestained MW markers.  Other detection systems will yield similar results.

VEGF-A Antibody - 200-401-B74S from Rockland Immunochemicals Inc

Prices $95.00
Sizes 25 µl
Host Rabbit
Clonality Polyclonal
Western blot using Rockland's Protein-A Purified anti-bovine VEGF-A antibody shows detection of recombinant bovine VEGF-A at 17-19.2 kDa (arrowhead).   Approximately 2 µg of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane.   The membrane was blocked using 3% BSA (p/n BSA-30) diluted 1:10.  The primary antibody was used at a 1:333 dilution and was incubated with the blot for 2h at room temperature.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10.  Molecular weight estimation was made by comparison to prestained MW markers.  Other detection systems will yield similar results.

Bovine VEGF-A Polyclonal Antibody - PA0146B-100 from Kingfisher Biotech

Host Bovine
Clonality Polyclonal

Bovine VEGF-A Polyclonal Antibody - PB0276B-100 from Kingfisher Biotech

Prices $300.00
Sizes 100 µg
Host Bovine
Clonality Polyclonal

Bovine VEGF-A Polyclonal Antibody - Biotinylated - PBB0279B-050 from Kingfisher Biotech

Prices $300.00
Sizes 50 µg
Host Bovine
Clonality Polyclonal

Vascular Endothelial Growth Factor A Antibody - abx104822 from Abbexa

Host Rabbit
Clonality Polyclonal

Vascular Endothelial Growth Factor A, Polyclonal Antibody - MBS2027951 from MyBioSource

Prices $140.00, $170.00, $250.00, $340.00, $530.00, $1,275.00
Sizes 10 µg, 20 µg, 50 µg, 100 µg, 200 µg, 1 mg
Host Rabbit
Clonality Polyclonal

VEGF-A Antibody - AHP2382 from AbD Serotec

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Vascular Endothelial Growth Factor A (VEGFA) Polyclonal Antibody - CAU28473 from Biomatik

Prices $120.00, $280.00, $401.00, $641.00
Sizes 10 μg, 50 μg, 100 μg, 200 μg
Host Rabbit
Clonality Polyclonal

Anti-Bovine-VEGF-A-Antibody - ABD13924 from Abgent, a WuXi AppTec company

Clonality Polyclonal

Recombinant-Bovine-VEGF-A - ABD14303 from Abgent, a WuXi AppTec company

VEGF-A, Polyclonal Antibody - MBS224849 from MyBioSource

Prices $525.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

VEGF-A, Polyclonal Antibody - MBS224910 from MyBioSource

Prices $525.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

VEGF-A antibody - orb344659 from Biorbyt

Host Rabbit
Clonality Polyclonal
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