Immunohistochemistry of VEGF staining of Paraffin Sections of Bovine follicules using AP02022SU VEGF antibody. Antigen retrieval was performed by heating the dewaxed sections 4x to 95°C for 5 min. The section was then treated with Hydrogen Peroxide (1%) in methanol for 30 min to block endogenous peroxidase. The section was incubated with AP02022SU (1/300) overnight, followed by staining with ABC method. DAB in 0.0006% Hydogen Peroxide/0.05M Tris buffer (pH7.6) was used as the chromogen. This antibody stains the cytoplasm of granulosa and theca cells of mature follicules. Scale bar: 100 µm. Berisha B et al. (2002) J. Endocrinol. 167(3): 371-382.