ANO1

Gene Symbol ANO1
Entrez Gene 55107
Alt Symbol DOG1, ORAOV2, TAOS2, TMEM16A
Species Human
Gene Type protein-coding
Description anoctamin 1, calcium activated chloride channel
Other Description Ca2+-activated Cl- channel|anoctamin-1|discovered on gastrointestinal stromal tumors protein 1|oral cancer overexpressed 2|transmembrane protein 16A (eight membrane-spanning domains)|tumor-amplified and overexpressed sequence 2
Swissprots Q5XXA6 Q8IYY8 A8KAM3 Q8N7V3
Accessions EAW74757 EAW74758 EAW74759 Q5XXA6 AB845669 BAO49755 AI694083 AK001123 BAA91513 AK097619 BAC05123 AK293088 BAF85777 AK303971 BAH14087 AL833582 AY728143 AAU82085 BC027590 AAH27590 BC033036 AAH33036 CF593428 DB445158 DB450993 HQ418153 ADP09411 XM_006718602 XP_006718665 XM_006718604 XP_006718667 XM_006718605 XP_006718668 XM_011545121 XP_011543423 XM_011545122 XP_011543424 XM_011545123 XP_011543425 XM_011545124 XP_011543426 XM_011545125 XP_011543427 XM_011545126 XP_011543428 XM_011545127 XP_011543429 XM_011545128 XP_011543430 XM_011545129 XP_011543431 XM_011545130 XP_011543432 XM_011545131 XP_011543433 NM_018043 NP_060513 NR_030691
Function Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. {ECO:0000269|PubMed:20056604, ECO:0000269|PubMed:21984732, ECO:0000269|PubMed:22178883, ECO:0000269|PubMed:22946059}.
Subcellular Location Cell membrane; Multi-pass membrane protein. Cytoplasm. Note=Cytoplasmic localization seen in neoplastic cells of head and neck squamous cell carcinoma (HNSCC) tumors.
Tissue Specificity Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles. {ECO:0000269|PubMed:15215166, ECO:0000269|PubMed:16906560}.

Anoctamin 1 (D1M9Q) Rabbit mAb - 14476 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1M9Q
Western blot analysis of extracts from various tissues and cell lines using Anoctamin 1 (D1M9Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

TMEM16A (C-5) - sc-377115 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TMEM16A (C-5): sc-377115. Western blot analysis of TMEM16A expression in human skeletal muscle (A) and human liver (B) tissue extracts.

TMEM16A (H-41) - sc-135235 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

TMEM16A (S-20) - sc-69343 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TMEM16A (S-20): sc-69343. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.

Anti-TMEM16A antibody - ab53212 from Abcam

Prices $382.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab53212 (2µg/ml) staining TMEM16A in human liver (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of hepatocyte cell membrane.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-TMEM16A antibody - ab72984 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab72984 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72984, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TMEM16A antibody - ab84915 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-TMEM16A antibody (ab84915) at 1 µg/ml + Brain (Human) Membrane Lysate - adult normal tissue (ab29456) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-TMEM16A antibody - ab84115 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TMEM16A antibody (ab84115) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-TMEM16A antibody [DG1/447] - ab190721 from Abcam

Prices $379.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone DG1447
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastrointestinal stromal tumor tissue labeling TMEM16A with ab190721 at 1 µg/ml.

Anti-TMEM16A antibody [DG1/447 + DOG-1.1] - ab200486 from Abcam

Prices $359.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone DG1/447 + DOG-1.1
Immunohistochemical analysis of paraffin-embedded Human gastrointestinal stromal tumor tissue, labeling TMEM16A using ab200486 at 1 μg/ml. Zoom 20X.

Anti-TMEM16A antibody [DOG1.1] - ab111160 from Abcam

Prices $376.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
Clone DOG1.1
ab111160 staining TMEM16A in Formalin-fixed, Paraffin-embedded Gastrointestinal stromal tumor tissue by Immunohistochemistry.

Anti-TMEM16A antibody [EPR10643(2)] - ab190803 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10643(2)
Immunohistochemical analysis of paraffin embedded Human gastrointestinal stromal tumor tissue sections labeling TMEM16A using ab190803 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Anti-TMEM16A antibody, prediluted - ab53213 from Abcam

Prices $394.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab53213 staining TMEM16A in human gastrointestinal stromal tumor sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with undiluted ab53213 for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.See Abreview

Anti-TMEM16A antibody [SP31] - ab64085 from Abcam

Prices $384.00
Sizes 500 µl
Host Rabbit
Clonality Monoclonal
Clone SP31
Immunohistochemical analysis of TMEM16A expression in paraffin embedded formalin fixed Human GIST tumor using 1/100 ab64085.

Anti-TMEM16A antibody [SP31], prediluted - ab64087 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Monoclonal
Clone SP31
Immunohistochemical analysis of TMEM16A expression in paraffin embedded formalin fixed human GIST tumor section, using ready to use ab64087.

Anti-TMEM16A antibody - C-terminal - ab191040 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TMEM16A antibody - C-terminal (ab191040) at 0.5 µg/mlLane 1 : Rat liver tissue lysateLane 2 : Rat skeletal muscle tissue lysateLysates/proteins at 40 µg per lane.

Anti-ANO1 / DOG1 / TMEM16A Antibody (N-Terminus) - LS-C144845 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ANO1 / DOG1 / TMEM16A Antibody (clone DOG1.1) - LS-C153076 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone DOG1.1

Anti-ANO1 / DOG1 / TMEM16A Antibody (N-Terminus) - LS-C153378 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ANO1 / DOG1 / TMEM16A Antibody - LS-C50491 from LifeSpan Bioscience

Prices $530.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of Human Gastrointestinal Stromal Tumor stained with anti-DOG-1 antibody

Anti-ANO1 / DOG1 / TMEM16A Antibody (N-Terminus) - LS-C108600 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 ug/ml.

Anti-ANO1 / DOG1 / TMEM16A Antibody (aa453-482, AP) - LS-C251377 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ANO1 / DOG1 / TMEM16A Antibody (aa453-482, APC) - LS-C251378 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ANO1 / DOG1 / TMEM16A Antibody (aa921-970) - LS-C199661 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HeLa cells, using TM16A Antibody. The sample on the right was incubated with synthetic peptide.

Anti-ANO1 / DOG1 / TMEM16A Antibody (aa453-482, Biotin) - LS-C251379 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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