Anti-TMEM16A antibody
| Name | Anti-TMEM16A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53212 |
| Prices | $382.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide of human TMEM16A protein |
| Description | Rabbit Polyclonal |
| Gene | ANO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab53212 (2µg/ml) staining TMEM16A in human liver (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of hepatocyte cell membrane.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab53212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Analysis of TMEM16A expression was performed in frozen liver sections (10 µm) from normal and 7-day bile duct-ligated rats and mice.Sections were defrosted at room temperature and fixed in 4% paraformaldehyde (1× PBS), permeabilized in PBST (1× PBS with 0.2% Triton), and blocked in 4% BSA (in PBST) for 1 hour at room temperature. Samples were then incubated with ab53212 at a 1/100 dilution, diluted in 1% BSA. Sections were incubated overnight at 4 °C and washed three times for 10 minutes each with 1× PBST at room temperature. Sections were incubated with Dylight 488 conjugated donkey anti-rabbit secondary at a 1/600 dilution for 2 hours at room temperature protected from light. Following incubation, the slides were washed in PBST at room temperature and coverslipped with Antifade gold and DAPI. Images were visualized using an Olympus IX-71 confocal microscope.Scale bar, 10 µm.
Product References
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Identification and functional characterization of TMEM16A, a Ca2+-activated Cl- - Identification and functional characterization of TMEM16A, a Ca2+-activated Cl-
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ETV1 is a lineage survival factor that cooperates with KIT in gastrointestinal - ETV1 is a lineage survival factor that cooperates with KIT in gastrointestinal
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Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse - Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse
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Genomewide mRNA profiling of esophageal squamous cell carcinoma for - Genomewide mRNA profiling of esophageal squamous cell carcinoma for
Kashyap MK, Marimuthu A, Kishore CJ, Peri S, Keerthikumar S, Prasad TS, Mahmood R, Rao S, Ranganathan P, Sanjeeviah RC, Vijayakumar M, Kumar KV, Montgomery EA, Kumar RV, Pandey A. Cancer Biol Ther. 2009 Jan;8(1):36-46. Epub 2009 Jan 1.
Regulation of TMEM16A chloride channel properties by alternative splicing. - Regulation of TMEM16A chloride channel properties by alternative splicing.
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