BRCA2

Gene Symbol BRCA2
Entrez Gene 675
Alt Symbol BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11
Species Human
Gene Type protein-coding
Description breast cancer 2, early onset
Other Description BRCA1/BRCA2-containing complex, subunit 2|Fanconi anemia group D1 protein|breast and ovarian cancer susceptibility gene, early onset|breast cancer 2 tumor suppressor|breast cancer type 2 susceptibility protein
Swissprots Q13879 O00183 Q5TBJ7 O15008 P51587
Accessions AAG32681 AAG32682 AAG46030 AAK29432 AAN28944 AAN61409 AAN61410 AAN61411 AAN61412 AAN61413 AAN61414 AAN61415 AAN61416 AAN61417 AAN61418 AAN61419 AAN61420 AAN61421 AAN61422 AAN61426 AAN61427 AAN61428 AAN61429 AAN61430 AAN61431 AAN61432 AAN61433 AAN61434 AAN61435 AAN61436 AAN61437 AAQ97181 AAZ22546 ABJ90481 ACD01217 AIC83529 AIC83530 AIC83531 AIC83532 AIC83533 AIC83534 AIC83535 AIC83536 AIC83537 AIC83538 AIC83543 AIC83560 AIC83561 AIC83562 AIC83563 AIC83564 AIC83565 AIC83566 AIC83567 AIC83568 AIC83569 AIC83570 AIC83583 AIC83584 AIC83585 AIC83586 AIC83587 AIC83588 AIY60806 AKE50582 AKE50583 AKE50584 AKE50585 AKE50586 AKE50587 AKE50588 AKE50589 AKE50590 AKE50591 AKE50592 AKE50593 AKE50594 AKE50595 CAA64484 CAA97728 CAA98995 EAX08499 EAX08500 EAX08501 P51587 BC026160 BC047568 DQ897648 ABI74674 GU014835 ACX32322 JX480468 AFU88810 JX480469 AFU88811 JX480470 AFU88812 JX480471 AFU88813 JX480472 AFU88814 JX480473 AFU88815 JX480474 AFU88816 JX480475 AFU88817 JX480476 AFU88818 JX480477 AFU88819 JX480478 AFU88820 U43746 AAB07223 XM_011535203 XP_011533505 XM_011535204 XP_011533506 XM_011535205 XP_011533507 NM_000059 NP_000050
Function Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) s
Subcellular Location Nucleus {ECO:0000305|PubMed:21276791}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:21276791}.
Tissue Specificity Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
Top Pathways Homologous recombination, Pancreatic cancer, Fanconi anemia pathway

BRCA2 Antibody - 9012 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from human (HeLa), murine (SV-T2) and bovine (BAEC) cells using BRCA2 Antibody.

BRCA2 (H-300) - sc-8326 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRCA2 (H-300): sc-8326. Western blot analysis of BRCA2 expression in HeLa nuclear extract.

BRCA2 (N-19) - sc-1819 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BRCA2 (N-19): sc-1819. Western blot analysis of BRCA2 expression in ZR-75-1 (A), 293T (B), MDA-MB-231 (C) and U-2 OS (D) whole cell lysates.

BRCA2 (C-19) - sc-1817 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BRCA2 (C-19): sc-1817. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear and cytoplasmic staining of Purkinje cells and cytoplasmic staining of cells in granular layer and cells in molecular layer.

BRCA2 (I-17) - sc-1818 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BRCA2 (I-17): sc-1818. Western blot analysis of BRCA2 expression in HeLa nuclear extract (A) and A-431 whole cell lysate (B).

BRCA2 (H-299) - sc-28235 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRCA2 (H-299): sc-28235. Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.

BRCA2 (C-15) - sc-1816 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

BRCA2 (T-18) - sc-21230 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BRCA2 (T-18): sc-21230. Immunofluorescence staining of methanol-fixed ZR-75-1 cells showing nuclear and cytoplasmic localization.

BRCA2 (V-19) - sc-21177 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-BRCA2 antibody - ab27976 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab27976 staining BRCA2 in a xenograft MDA-MB-231 of human breast cancer by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% BSA for 30 minutes at 25°C and then incubated with ab27976 at a 1/50 dilution for 16 hours at 25°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.See Abreview

Anti-BRCA2 antibody - ab123491 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BRCA2 antibody (ab123491) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa at 50 µg/mlLane 2 : Whole cell lysate from HeLa at 15 µg/mlLane 3 : Whole cell lysate from 293T at 50 µg/mlLane 4 : Whole cell lysate from Jurkat at 50 µg/mldeveloped using the ECL technique

Anti-BRCA2 antibody - ab9143 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-BRCA2 antibody - ab90541 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BRCA2 antibody (ab90541)Lane 1 : Capan 1Lane 2 : HeLa

Anti-BRCA2 antibody - ab191137 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-BRCA2 antibody (ab191137) at 0.5 µg/mlLane 1 : Rat Testis Tissue LysateLane 2 : SKOV Whole Cell LysateLane 3 : MCF7 Whole Cell LysateLane 4 : A549 Whole Cell LysateLane 5 : PC12 Whole Cell LysateLysates/proteins at 40 µg per lane.

Anti-BRCA2 antibody - ab75335 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BRCA2 antibody - ab110967 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 1/50 BRCA2 antibody (ab110967). Right image shows sample treated with blocking peptide.

Anti-BRCA2 antibody [3E6] - ab97 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3E6
Overlay histogram showing HeLa cells stained with ab97 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab97, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-BRCA2 antibody [9D3] - ab96 from Abcam

Host Mouse
Clonality Monoclonal
Clone 9D3

Anti-BRCA2 antibody (Agarose) - ab1292 from Abcam

Host Rabbit
Clonality Polyclonal

BRCA2 Antibody (234403) - MAB2476 from Novus Biologicals

Prices $99.00, $259.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 234403
Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRCA2 at approximately 380 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BRCA2 Antibody (5F6) - NBP1-41189 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5F6

BRCA2 Antibody (9D3) - NBP1-41187 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9D3
Immunohistochemistry-Paraffin: BRCA2 Antibody (9D3) [NBP1-41187] - Paraffin-embedded Hepatoma, using antibody at 1:200 dilution.

BRCA2 Antibody (3E6) - NB600-445 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E6
Immunocytochemistry/Immunofluorescence: BRCA2 Antibody (3E6) [NB600-445] - Analysis of paraformaldehyde-fixed HeLa, using BRCA2 antibody at 1:100 dilution.

BRCA2 Antibody - NB100-65084 from Novus Biologicals

Prices $299.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

BRCA2 Antibody - NBP1-51199 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: BRCA2 Antibody [NBP1-51199] Immunohistochemistry (IHC) analyzes of BRCA2 (N60) pAb in paraffin-embedded human breast carcinoma tissue.
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