CFL1

Gene Symbol CFL1
Entrez Gene 1072
Alt Symbol CFL, HEL-S-15
Species Human
Gene Type protein-coding
Description cofilin 1 (non-muscle)
Other Description 18 kDa phosphoprotein|cofilin, non-muscle isoform|cofilin-1|epididymis secretory protein Li 15|p18
Swissprots P23528 Q9UCA2 Q53Y87 B3KUQ1
Accessions EAW74448 EAW74449 EAW74450 EAW74451 EAW74452 P23528 AK097690 BAG53513 AK303614 BAG64624 BC011005 AAH11005 BC012265 AAH12265 BC012318 AAH12318 BC018256 AAH18256 BC024975 BT006846 AAP35492 CB850882 D00682 BAA00589 D28345 BAA05711 DQ891769 ABM82695 EU176624 ABW03425 EU794603 ACJ13657 HM370399 U21909 AAA64501 X95404 CAA64685 NM_005507 NP_005498
Function Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. {ECO:0000269|PubMed:15580268, ECO:0000269|PubMed:21834987, ECO:0000269|PubMed:23633677}.
Subcellular Location Nucleus matrix {ECO:0000269|PubMed:15580268}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:15580268}. Cell projection, ruffle membrane {ECO:0000269|PubMed:15580268}; Peripheral membrane protein {ECO:0000269|PubMed:15580268}; Cytoplasmic side {ECO:0000269|PubMed:15580268}. Cell projection, lamellipodium membrane {ECO:0000269|PubMed:15580268}; Peripheral membrane protein {ECO:0000269|PubMed:15580268}; Cytoplasmic side {ECO:0000269|PubMed:15580268}. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Tissue Specificity Widely distributed in various tissues.
Top Pathways Axon guidance, Fc gamma R-mediated phagocytosis, Pertussis, Regulation of actin cytoskeleton

Cofilin (D3F9) XP ® Rabbit mAb (HRP Conjugate) - 8503 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3F9
Western blot analysis of extracts from COS-7 and HeLa cells using Cofilin (D3F9) XP ® Rabbit mAb (HRP Conjugate).

Phospho-Cofilin (Ser3) Antibody - 3311 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or H2O2-treated, using Phospho-Cofilin (Ser3) Antibody (A and B) or Cofilin Antibody #3312 (C and D). Membranes (B and D) were treated with alkaline phosphatase (CIP) to demonstrate the phospho-specificity the antibody.

Phospho-Cofilin (Ser3) (77G2) Rabbit mAb - 3313 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 77G2
Western blot analysis of COS cells, untreated or λ phosphatase-treated, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (upper) or Cofilin Antiobdy #3312 (lower).

Cofilin (D3F9) XP ® Rabbit mAb - 5175 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3F9
Western blot analysis of extracts from various cell types using Cofilin (D3F9) XP ® Rabbit mAb.

Cofilin (D59) Antibody - 3318 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using Cofilin (D59) Antibody.

Cofilin Antibody - 3312 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using Cofilin Antibody.

Cofilin (E-8) - sc-376476 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cofilin (E-8): sc-376476. Western blot analysis of Cofilin 1 expression in non-transfected: sc-117752 (A) and human Cofilin 1 transfected: sc-112308 (B) 293T whole cell lysates.

Cofilin (N-19) - sc-8441 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cofilin (N-19): sc-8441. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

Cofilin (FL-166) - sc-33779 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cofilin (FL-166): sc-33779. Western blot analysis of Cofilin expression in non-transfected 293T: sc-117752 (A), human Cofilin 1 transfected 293T: sc-112308 (B) and HeLa (C) whole cell lysates.

Cofilin 1 (H-12) - sc-32158 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cofilin 1 (H-12): sc-32158. Western blot analysis of Cofilin 1 expression in Jurkat whole cell lysate.

Cofilin 1 (5) - sc-53934 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cofilin 1 (5): sc-53934. Western blot analysis of Cofilin 1 expression in HeLa (A), K-562 (B) and Jurkat (C) whole cell lysates.

Cofilin 1 (L-13) - sc-32157 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cofilin 1 (L-13): sc-32157. Western blot analysis of Cofilin 1 expression in Jurkat (A), HeLa (B), HEK293 (C), MCF7 (D), NIH/3T3 (E) and PC-12 (F) whole cell lysates.

p-Cofilin 1 (E-5) - sc-271921 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Cofilin 1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human Cofilin 1 transfected: sc-172171 (B,E) and lambda protein phosphatase treated human Cofilin 1 transfected: sc-172171 (C,F) 293T whole cell lysates. Antibodies tested include p-Cofilin 1 (E-5): sc-271921 (A,B,C) and Cofilin (FL-166): sc-33779 (D,E,F).

p-Cofilin 1 (F-11) - sc-365882 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Cofilin 1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human Cofilin 1 transfected: sc-112308 (B,E) and lambda protein phosphatase (sc-200312A) treated human Cofilin 1 transfected: sc-112308 (C,F) 293T whole cell lysates. Antibodies tested include p-Cofilin 1 (F-11): sc-365882 (A,B,C) and Cofilin (FL-166): sc-33779 (D,E,F).

p-Cofilin 1 (H-2) - sc-271923 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Cofilin 1 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include p-Cofilin 1 (H-2): sc-271923 (A,B) and Cofilin 1 (5): sc-53934 (C,D).

Anti-Cofilin (phospho S3) antibody - ab131274 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cofilin (phospho S3) antibody (ab131274) at 1/500 dilutionLane 1 : COLO205 cell extract with blocking peptideLane 2 : COLO205 cell extract

Anti-Cofilin (phospho S3) antibody - ab100836 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab100836 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml,.

Anti-Cofilin (phospho S3) antibody - ab47281 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cofilin (phospho S3) antibody (ab47281) at 1/500 dilutionLane 1 : Extracts from COLO205 cells serum starvedLane 2 : Extracts from COLO205 cells Lysates/proteins at 30 µg per lane.

Anti-Cofilin (phospho S3) antibody [3E8.A9] - ab184680 from Abcam

Prices $378.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3E8.A9
All lanes : Anti-Cofilin (phospho S3) antibody [3E8.A9] (ab184680) at 1/100 dilutionLane 1 : HeLa cell lysateLane 2 : BSA negative control

Anti-Cofilin (phospho Y139) antibody - ab194783 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Cofilin (phospho Y139) with ab194783 at 1/50 dilution.

Anti-Cofilin (phospho Y140 + S156) antibody - ab76215 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Cofilin (phospho Y140 + S156) antibody - ab77518 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Cofilin 1 + 2 (phospho Y88) antibody - ab194873 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Cofilin 1 + 2 (phospho Y88) antibody (ab194873) at 1/500 dilution + Mouse heart tissue lysate

Anti-Cofilin 1 + 2 antibody - ab131519 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Cofilin 1 + 2 antibody (ab131519) at 1/500 dilution + MDA cell extract

Anti-Cofilin antibody - ab42824 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cofilin antibody (ab42824) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
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