Western blot analysis of Cofilin 1 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include p-Cofilin 1 (H-2): sc-271923 (A,B) and Cofilin 1 (5): sc-53934 (C,D).
BVT 948: sc-203536. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), BVT 948 treated K-562 (C) and BVT 948 and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by BVT 948 in lane D.
Endothall: sc-201325. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), Endothall treated K-562 (C) and Endothall and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by Endothall in lane D.
Salubrinal: sc-202332. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), Salubrinal treated K-562 (C) and Salubrinal and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by Salubrinal in lane D.
Sodium metavanadate: sc-251034. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), Sodium metavanadate treated K-562 (C) and Sodium metavanadate and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by Sodium metavanadate in lane D.
Sodium Orthovanadate: sc-3540. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), Sodium Orthovanadate treated K-562 (C) and Sodium Orthovanadate and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by Sodium Orthovanadate in lane D.
EGTA: sc-3593. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), EGTA treated K-562 (C) and EGTA and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by EGTA in lane D.
Phenylarsine Oxide (PAO): sc-3521. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), Phenylarsine Oxide (PAO) treated K-562 (C) and Phenylarsine Oxide (PAO) and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by Phenylarsine Oxide (PAO) in lane D.
bpV(bipy): sc-221376. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), bpV(bipy) treated K-562 (C) and bpV(bipy) and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by bpV(bipy) in lane D.
bpV(phen): sc-221378. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), bpV(phen) treated K-562 (C) and bpV(phen) and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by bpV(phen) in lane D.
bpV(pic): sc-221379. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), bpV(pic) treated K-562 (C) and bpV(pic) and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by bpV(pic) in lane D.
NSC 95397: sc-203654. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), NSC 95397 treated K-562 (C) and NSC 95397 and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by NSC 95397 in lane D.
Phosphatase Inhibitor Cocktail A: sc-45044. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), Phosphatase Inhibitor Cocktail A treated K-562 (C) and Phosphatase Inhibitor Cocktail A and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by Phosphatase Inhibitor Cocktail A in lane D.
Phosphatase Inhibitor Cocktail B: sc-45045. Western blot analysis of Cofilin 1 phosphorylation in K-562 (A), lambda phosphatase (sc-200312) treated K-562 (B), Phosphatase Inhibitor Cocktail B treated K-562 (C) and Phosphatase Inhibitor Cocktail B and lambda phosphatase treated K-562 (D). Antibody tested: p-Cofilin 1 (H-2): sc-271923. Note inhibition of lambda phosphatase by Phosphatase Inhibitor Cocktail B in lane D.