ATG5

Gene Symbol ATG5
Entrez Gene 9474
Alt Symbol APG5, APG5-LIKE, APG5L, ASP, hAPG5
Species Human
Gene Type protein-coding
Description autophagy related 5
Other Description ATG5 autophagy related 5 homolog|apoptosis-specific protein|autophagy protein 5
Swissprots Q9H1Y0 Q9HCZ7 Q5JVR2 Q9H2B8 O60875 Q68DI4
Accessions EAW48414 EAW48415 EAW48416 EAW48417 EAW48418 Q9H1Y0 AF293841 AAG44955 AK001899 BAG50990 AK299577 BAH13072 AK315800 BC002699 AAH02699 BC093011 AAH93011 BM831472 BP297166 BX537904 CAD97890 CR749386 CAH18236 EU283338 EU283339 ABX79918 HQ447996 ADQ32477 HY168998 JQ918353 AGC52701 JQ918354 AGC52702 JQ924061 AGC52703 Y11588 CAA72327 XM_011536263 XP_011534565 NM_001286106 NP_001273035 NM_001286107 NP_001273036 NM_001286108 NP_001273037 NM_001286111 NP_001273040 NM_004849 NP_004840 NR_104402 NR_104403
Function Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12- ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in
Subcellular Location Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. {ECO:0000250}.
Tissue Specificity Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Top Pathways RIG-I-like receptor signaling pathway, Regulation of autophagy

Atg5 (D5F5U) Rabbit mAb - 12994 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5F5U
Western blot analysis of extracts from various cell lines using Atg5 (D5F5U) Rabbit mAb.

Atg5 (D5G3) Rabbit mAb - 9980 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5G3
Western blot analysis of extracts from various cell lines using Atg5 (D5G3) Rabbit mAb.

Atg5 Antibody - 2630 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PANC1, HeLa and HT1080 cell lines using Atg5 Antibody.

Atg5 (D6M7Q) Rabbit mAb - 13007 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D6M7Q
Western blot analysis of extracts from various cell lines using Atg5 (D6M7Q) Rabbit mAb.

Atg5 (D1G9) Rabbit mAb - 8540 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D1G9

APG5 (C-1) - sc-133158 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
APG5 (C-1): sc-133158. Western blot analysis of APG5 expression in non-transfected: sc-117752 (A) and mouse APG5 transfected: sc-118471 (B) 293T whole cell lysates.

APG5 (FL-275) - sc-33210 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
APG5 (FL-275): sc-33210. Western blot analysis of APG5 expression in SH-SY5Y whole cell lysate.

APG5 (N-18) - sc-8666 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
APG5 (N-18): sc-8666. Western blot analysis of APG5 expression in SH-SY5Y (A), HEL 92.1.7 (B), Raji (C) and  CCRF-CEM (D) whole cell lysates.

APG5 (C-20) - sc-8667 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
APG5 (C-20): sc-8667. Western blot analysis of APG5 expression in SH-SY5Y (A), HEL 92.1.7 (B), Raji (C), THP-1 (D), HeLa (E) and HT-1080 (F) whole cell lysates.

Anti-APG5L/ATG5 antibody - ab78073 from Abcam

Prices $391.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of APG5L/ATG5 in formalin fixed paraffin embedded kidney tissue using 5 µg/ml of ab78073.

Anti-APG5L/ATG5 antibody - ab77580 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-APG5L/ATG5 antibody (ab77580) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-APG5L/ATG5 antibody - ab106551 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
Lane 1 :  Anti-APG5L/ATG5 antibody (ab106551) at 1 µg/mlLane 2 :  Anti-APG5L/ATG5 antibody (ab106551) at 2 µg/mlLane 1 : Mouse spleen tissue lysateLane 2 : Mouse spleen tissue lysateLysates/proteins at 15 µg per lane.

Anti-APG5L/ATG5 antibody - ab85742 from Abcam

Host Sheep
Clonality Polyclonal

Anti-APG5L/ATG5 antibody - ab64227 from Abcam

Host Rabbit
Clonality Polyclonal
ab64227 staining mouse Testis tissue sections by immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and blocked in 1% serum for 10 minutes at   20°C. The primary antibody was diluted 1/800 and incubated with sample for 20 hours at 20°C. The biotin conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary.See Abreview

Anti-APG5L/ATG5 antibody - ab64217 from Abcam

Host Sheep
Clonality Polyclonal

Anti-APG5L/ATG5 antibody - ab56646 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56646 APG5L/ATG5 antibody at 1ug/ml.

Anti-APG5L/ATG5 antibody [EPR1755(2)] - ab108327 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1755(2)
All lanes : Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : HeLa cell lysateLane 3 : HT-1080 cell lysateLane 4 : Human fetal kidney cell lysateLane 5 : C6 cell lysateLane 6 : Raw264.7 cell lysateLane 7 : PC-12 cell lysateLane 8 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.

Anti-APG5L/ATG5 antibody [EPR1755(2)] (HRP) - ab199560 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1755(2)
All lanes : Anti-APG5L/ATG5 antibody [EPR1755(2)] (HRP) (ab199560) at 1/5000 dilutionLane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell LysateLane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-APG5L/ATG5 antibody [EPR4797] - ab109490 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4797
All lanes : Anti-APG5L/ATG5 antibody [EPR4797] (ab109490) at 1/1000 dilutionLane 1 : THP1 cell lysateLane 2 : Raji cell lysateLane 3 : HeLa cell lysateLane 4 : HT1080 cell lysateLysates/proteins at 10 µg per lane.

Anti-APG5 / ATG5 Antibody (C-Terminus) IHC-plus™ - LS-B4678 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ATG5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4678 concentration 5 ug/ml.

Anti-APG5 / ATG5 Antibody (aa1-30) IHC-plus™ - LS-B11793 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-APG5 / ATG5 Antibody - LS-C147011 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-APG5 / ATG5 Antibody (N-Terminus, DY550) - LS-C141452 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-APG5 / ATG5 Antibody (N-Terminus) - LS-C53246 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 50 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of ATG5 in Human Spleen cells with ATG5 antibody at 20 ug/ml.

Anti-APG5 / ATG5 Antibody (clone 4E7) - LS-C139447 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4E7
ATG5 monoclonal antibody (M09), clone 4E7. Western blot of ATG5 expression in Jurkat.
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