Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

Fluorescent image of U251 cells stained with ATG5 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

The anti-APG5L antibody is used in Western blot to detect APG5L in mouse brain tissue lysate.

Western blot of APG5L (arrow) using rabbit polyclonal APG5L Antibody (D3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.

Western blot of APG5L Antibody (D3) (arrow) using rabbit polyclonal APG5L Antibody (D3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

APG5L Antibody (D3) western blot of HeLa cell line lysates (35 ug/lane). The APG5L antibody detected the APG5L protein (arrow).