FA Functional Assay, Bioactive



Complex I Enzyme Activity Dipstick Assay Kit - ab109720 from Abcam

Prices $343.00
Sizes 48 tests
An example using ab109720 to measure Complex I activity in human fibroblast samples. Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 30 µg of fibroblast protein extract.

Complex IV Human Enzyme Activity Dipstick Assay Kit - ab109876 from Abcam

Prices $354.00, $627.00
Sizes 30 tests, 90 tests
Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

Complex IV Rodent Enzyme Activity Dipstick Assay Kit - ab109878 from Abcam

Prices $343.00, $627.00
Sizes 30 tests, 90 tests
Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit - ab109882 from Abcam

Prices $343.00, $627.00
Sizes 30 tests, 90 tests
Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) - ab174097 from Abcam

Category Cellular Assay
Prices $520.00
Sizes 100 tests
Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by colorimetric reading. Please not this data is example data only.

2X LDL/VLDL Precipitation Buffer - ab105138 from Abcam

Category Cellular Assay
Prices $232.00
Sizes 10 ml

5-formylcytosine DNA Quantification Kit (Colorimetric) - ab156905 from Abcam

Category Cellular Assay
Prices $402.00, $700.00
Sizes 48 tests, 96 tests
5-formylcytosine standard control was added into the assay wells at different concentrations and then measured with Abcam's 5-formylcytosine DNA Quantification Kit (Colorimetric) (ab156905)

6-Phosphofructokinase Activity Assay Kit (Colorimetric) - ab155898 from Abcam

Category Cellular Assay
Prices $690.00
Sizes 100 tests
Sensitivity < 1 mU/well
Range 2 nmol/well - 10 nmol/well
PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.
K562 cells (target cells) were labelled with CFSE and co-cultured with NK cells (effector cells) at the ratios indicated above each panel for four hours at 37°C. At the end of co-incubation, dead cells were labeled with 7-AAD. The percentage of K562 cell death was analyzed by flow cytometry. When cultured alone, there is little cell death (2%) on the K562 cell population. Addition of NK cells at a ratio as low as 6.25:1 caused a significant increase in K562 cell death (8.2%). At a ratio of NK cells to target cells of 25:1, there is 19.7% death of K562 cells.

7-Amino-4-trifluoromethylcoumarin (AFC) - ab129872 from Abcam

Category Cellular Assay
Prices $175.00
Sizes 100 mg
Structure of 7-Amino-4-trifluoromethylcoumarin (AFC)

Acetoacetate Assay Kit (Colorimetric) - ab180875 from Abcam

Category Cellular Assay
Prices $572.00
Sizes 100 tests
Sensitivity < 25 µM
Acetoacetate measured in urine samples showing concentration (micromolar).

Acetylcholinesterase Assay Kit (Colorimetric) - ab138871 from Abcam

Category Cellular Assay
Prices $278.00
Sizes 200 tests
Sensitivity 1 mU/ml
AChE measured in mouse tissue lysates showing quantity (mU) per mg protein of tested sample

Acetylcholinesterase Assay Kit (Fluorometric - Green) - ab138872 from Abcam

Category Cellular Assay
Prices $278.00
Sizes 200 tests
Sensitivity 0.1 mU/ml
Acetylcholinesterase dose response was measured in a solid black 96-well plate with ab138872 using a fluorescence microplate reader. As low as 0.01 mU/well of acetylcholinesterase can be detected with 20 minutes incubation (n=3).

Acetylcholinesterase Assay Kit (Fluorometric -Red) - ab138873 from Abcam

Category Cellular Assay
Prices $278.00
Sizes 200 tests
Sensitivity 0.1 mU/ml
AChE measured in cell lysates showing activity per 1 million cells 

Acid Phosphatase Assay Kit (Colorimetric) - ab83367 from Abcam

Category Cellular Assay
Prices $304.00
Sizes 500 tests
Sensitivity > 0.001 mM
Range 0.001 mM - 1 mM
p-Nitrophenol (pNP) standard curve

Acid Phosphatase Assay Kit (Fluorometric) - ab83370 from Abcam

Category Cellular Assay
Prices $304.00
Sizes 500 tests
Sensitivity 0.001 mU/ml
4-Methylumbelliferone (4-MU) Standard Curve.

Acidic Sphingomyelinase Assay Kit (Fluorimetric) - ab190554 from Abcam

Category Cellular Assay
Prices $369.00
Sizes 200 tests
Sensitivity 1 U/ml

Aconitase Assay Kit - ab83459 from Abcam

Category Cellular Assay
Prices $464.00
Sizes 100 tests
Aconitase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.

Aconitase Enzyme Activity Microplate Assay Kit - ab109712 from Abcam

Category Cellular Assay
Prices $448.00
Sizes 96 tests
Figure 2. Using cellular fractionation kit ab109719, whole cells were separated into cytoplasmic, mitochondrial and nuclear fractions.

Adenosylhomocysteinase (AHCY) Activity Assay Kit (Fluorometric) - ab197002 from Abcam

Category Cellular Assay
Prices $725.00
Sizes 100 tests
Sensitivity > 1 µU
Typical Adenosine Standard Curve obtained following assay protocol.

Adipogenesis Assay Kit (Cell-Based) - ab133102 from Abcam

Category Cellular Assay
Prices $387.00
Sizes 1 kit
Lipid droplet accumulation in the differentiated 3T3-L1 cells visualized by Oil Red O Solution staining.

Adipogenesis Detection Kit - ab102513 from Abcam

Category Cellular Assay
Prices $381.00
Sizes 100 tests
Sensitivity > 0.2 nM
Range 0.2 nM - 10 nM
Glycerol measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

Adipolysis Assay Kit - ab133115 from Abcam

Category Cellular Assay
Prices $387.00
Sizes 1 kit
Differentiated 3T3-L1 cells were treated with increasing concentrations of isoproterenol for 48 hours and the culture supernatant was assayed for free glycerol. Top panel: glycerol release from undifferentiated (red) and differentiated (blue) 3T3-L1 cells as determined using Free Glycerol Assay Reagent. Bottom panel: isoproterenol dose response curve showing glycerol released after 24 hours versus isoproterenol concentration.

ADP Assay Kit (Colorimetric/Fluorometric) - ab83359 from Abcam

Category Cellular Assay
Prices $464.00
Sizes 100 tests
Sensitivity > 1 µM
ADP levels measured fluorometrically in cell culture supernatants (background signal subtracted, mean of duplicates; +/- SD).

ADP/ATP Ratio Assay Kit (Bioluminescent) - ab65313 from Abcam

Category Cellular Assay
Prices $572.00
Sizes 200 tests
Sensitivity < 100 cells/well
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