CD40 protein (Fc Chimera Active)
| Name | CD40 protein (Fc Chimera Active) |
|---|---|
| Supplier | Abcam |
| Catalog | ab83917 |
| Category | Protein |
| Prices | $302.00 |
| Sizes | 25 µg |
| Applications | SDS-PAGE WB FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Activity: The ED 50 of ab83917 is typically 1.0-1.5 ug/ml as measured by its ability to neutralize CD40 ligand mediated proliferation of T47-D cells. |
| SwissProt/Accession | P25942 |
| Gene | CD40 |
| Sequence | Theoretical Sequence EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDT WNR ETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVL HRSC SPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLV VQQAG TNKTDVVCGPQDRLRRSSNTKVDKKVEPKSCDKTHTCPPCPAPELLGG PSVFL FPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK PREEQ YNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP REPQVY TLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPV LDSDG SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| Supplier Page | Shop |
Product images
Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83917 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab83917 Lane 2: ab83917 treated with PNGase F to remove potential N-linked glycans Lane 3: ab83917 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
Anti-CD40 antibody ( ab13545 ) at 1/1000 dilution + CD40 protein (Fc Chimera Active) (ab83917) at 1 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080 ) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Observed band size : 72 kDa ( why is the actual band size different from the predicted? ) Exposure time : 8 minutes