Recombinant Human USP1/UAF1, His-tagged

Name Recombinant Human USP1/UAF1, His-tagged
Supplier Creative Biomart
Catalog USP1-161H
Category Protein
Species Reactivities Human
Nature Recombinant
Source Insect cells
Tag/Conjugation His
Purity >90% by SDS-PAGE
Bioactivity Deubiquitylase Enzyme Assay: The activity of 6His-USP1/UAF1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of 6His-USP1/UAF1 was compared confirming the deubiquitylating activity of 6His-USP1/UAF1.
SwissProt/Accession O94782
Gene USP1
Description Recombinant human USP1 [6His-tagged] / UAF1 [untagged] heterodimer were co-expressed using an insect cell.
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