Human Tau (phospho S214) peptide
| Name | Human Tau (phospho S214) peptide |
|---|---|
| Supplier | Abcam |
| Catalog | ab19123 |
| Category | Peptide |
| Applications | B/N |
| For Antibody | Anti-Tau (phospho S214) antibody |
| Nature | Synthetic |
| Purity | 70 - 90% by HPLC. |
| SwissProt/Accession | P10636 |
| Gene | MAPT |
| Sequence | Contains 4 Tau/MAP repeats. |
| Supplier Page | Shop |
Product images
ab10891 gave a positive result in ELISA against the immunising peptide (ab19123). This ELISA shows that ab10891 antibody has some cross-reactivity with the non-modified equivalent peptide ( ab23425 ) when used at high concentrations. Not yet tested in other applications.
All lanes : Anti-Tau (phospho S214) antibody ( ab10891 ) at 1/250 dilution Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Tau (phospho S214) peptide (ab19123) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 79 kDa Observed band size : 90 kDa ( why is the actual band size different from the predicted? ) Exposure time : 2 minutes