Name | Active human MMP2 full length protein |
---|---|
Supplier | Abcam |
Catalog | ab198429 |
Category | Protein |
Prices | $1,250.00 |
Sizes | 20 µg |
Applications | SDS-PAGE FA |
Species Reactivities | Human |
Nature | Recombinant |
Source | HEK 293 cells |
Tag/Conjugation | His tag C-Terminus |
Purity | >90% by SDS-PAGE. |
Bioactivity | Specific Activity: 511 pmole/min/µg. Activation: This is a proform enzyme and requires activation prior to testing activity. Dilute the enzyme to 100 ng/ml in a solution containing 50 mM HEPES, pH 7.4, 10 mM CaCl 2 , 0.05% Brij-35, and 1 mM APMA (amino-phenyl mercuric acetate). Incubate at 37ºC for 2 hours. Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393. |
SwissProt/Accession | P08253 |
Gene | MMP2 |
Residue | 30 to 660 |
Sequence | APSPIIKFPGDVAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQK FFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRII GYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHG DGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNAD GEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEAL FTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKY GFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRS DGKMWCAT TANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTY TKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDG IAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAP QEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKN KKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGG HSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGCHHHHHHHHHH |
Supplier Page | Shop |