| Bioactivity |
The effects of phospho ERK1/2 and non phospho ERK1/2 by ab113403 in THP1 cells. To examine the signal of phospho p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37°C 0, 30, 60, min, respectively by adding the recombinant protein (100 ng/ml) to the THP1 monocyte cell, which was maintained with serum starvation for 24 hrs. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP1 monocyte cell treatments over 0, 30, 60 mins, respectively. 2. The effects of phospho ERK1/2 and non phospho ERK1/2 by ab113403 in MCF10A cells. To examine the signal of phospho p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37°C over 0, 10, 30, 60 mins, respectively by adding the recombinant protein (500 ng/ml) to the MCF10A human breast epithelial cells, which was maintained with serum starvation for 24 hrs. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30, 60 mins, respectively.
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