TSC1

Gene Symbol TSC1
Entrez Gene 7248
Alt Symbol LAM, TSC
Species Human
Gene Type protein-coding
Description tuberous sclerosis 1
Other Description hamartin|tuberous sclerosis 1 protein|tumor suppressor
Swissprots Q5VVN5 B7Z897 Q92574
Accessions AAF61948 AAG02406 AAK12099 AAK60414 AAK60415 AAK60416 ABS18758 EAW88021 Q92574 AA215977 AB190910 BAD91314 AF013168 AAC51674 AK297326 BAH12548 AK299654 BAH13090 AK303030 BAH13883 AK308412 BC047772 AAH47772 BC070032 AAH70032 BC108668 AAI08669 BC121000 AAI21001 BC167824 AAI67824 BI463204 D87683 BAA13436 DA726552 KJ534953 AHW56593 N63914 XM_005272211 XP_005272268 XM_006717271 XP_006717334 XM_006717272 XP_006717335 XM_011518979 XP_011517281 NM_000368 NP_000359 NM_001162426 NP_001155898 NM_001162427 NP_001155899 NM_001008567
Function In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. {ECO:0000269|PubMed:12271141, ECO:0000269|PubMed:15340059}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:9809973}. Membrane {ECO:0000269|PubMed:9809973}; Peripheral membrane protein {ECO:0000269|PubMed:9809973}. Note=At steady state found in association with membranes.
Tissue Specificity Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
Top Pathways Insulin signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Choline metabolism in cancer, mTOR signaling pathway

Hamartin/TSC1 (1B2) Mouse mAb - 4963 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1B2
Western blot analysis of extracts from PC3 and SK-N-MC cells, using Hamartin/TSC1 (1B2) Mouse mAb.

Hamartin/TSC1 Antibody - 4906 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3, MDA-MB-468 and PC12 cells, untreated or lambda phosphatase-treated, using Hamartin/TSC1 Antibody.

Hamartin/TSC1 (D43E2) Rabbit mAb - 6935 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D43E2
Western blot analysis of extracts from wild type and TSC1 -/- MEF cells, HT-1080, and Rat2 cells using Hamartin/TSC1 (D43E2) Rabbit mAb.

hamartin (C-20) - sc-12082 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
hamartin (C-20): sc-12082. Western blot analysis of hamartin expression in MDA-MB-231 (A) and MDA-MB-468 (B) whole cell lysates.

hamartin (C-8) - sc-377386 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
hamartin (C-8): sc-377386. Western blot analysis of hamartin expression in SJRH30 (A) and IMR-32 (B) whole cell lysates.

hamartin (E-7) - sc-377387 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
hamartin (E-7): sc-377387. Western blot analysis of hamartin expression in SJRH30 (A) and IMR-32 (B) whole cell lysates.

hamartin (18) - sc-136436 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
hamartin (18): sc-136436. Western blot analysis of hamartin expression in WI-38 whole cell lysate.

hamartin (E-20) - sc-12080 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
hamartin (E-20): sc-12080. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing membrane and cytoplasmic staining of myocytes.

hamartin (H-300) - sc-13013 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

hamartin (N-17) - sc-12079 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-hamartin (Thr 417) - sc-130192 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Hamartin antibody - ab21632 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Hamartin antibody (ab21632) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µgSecondary Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Anti-Hamartin antibody - ab111981 from Abcam

Prices $384.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab111981, at 3.75µg/ml, staining Hamartin in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry.

Anti-Hamartin antibody - ab25882 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hamartin antibody (ab25882) at 1 µg/mlLane 1 : EL4 cell lysate with absence of blocking peptideLane 2 : EL4 cell lysate with presence of blocking peptide

Anti-Hamartin antibody - ab48028 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Hamartin antibody (ab48028) at 0.1 µg/ml + 35µg Human brain lysate in RIPA bufferSecondaryanti-goat-HRP at a 1/3000 dilutiondeveloped using the ECL technique

Anti-Hamartin antibody - ab25881 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-Hamartin antibody (ab25881) at 2 µg/mlLane 2 : Anti-Hamartin antibody (ab25881) at 4 µg/mlLane 1 : C2C12 cell lysate Lane 2 : C2C12 cell lysate

Anti-Hamartin antibody [3A2] - ab170164 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A2
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Hamartin with ab170164 at 1/150 dilution.

Anti-Hamartin antibody [EP318Y] - ab40872 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP318Y
Overlay histogram showing HeLa cells stained with ab40872 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40872, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Hamartin antibody - N-terminal - ab135111 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-Hamartin antibody - N-terminal (ab135111) at 1/500 dilution + HeLa total cell extracts

Anti-Hamartin / TSC1 Antibody (aa1150-1164) - LS-C313152 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Hamartin / TSC1 antibody. IHC(P): Rat Brain Tissue.

Anti-Hamartin / TSC1 Antibody (phospho-Ser1130, APC) - LS-C272319 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Hamartin / TSC1 Antibody (phospho-Ser1130, AP) - LS-C272318 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Hamartin / TSC1 Antibody (phospho-Ser1130, FITC) - LS-C272321 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Hamartin / TSC1 Antibody (phospho-Ser1130, Azide-free, HRP) - LS-C272322 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Hamartin / TSC1 Antibody (phospho-Ser1130, Biotin) - LS-C272320 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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