RHOA

Gene Symbol RHOA
Entrez Gene 395442
Alt Symbol -
Species Chicken
Gene Type protein-coding
Description ras homolog family member A
Other Description RhoA GTPase|ras homolog gene family, member A|transforming protein RhoA
Accessions AF098513 AAD12255 AJ294705 CAC08447 BX935572 U79757 AAC18962 NM_204704 NP_990035
Top Pathways Wnt signaling pathway, TGF-beta signaling pathway, Adherens junction, Tight junction, Focal adhesion

Anti-RhoA antibody - ab68826 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab68826 stained HeLa cells (ab150035). The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab68826 1/200 dilution overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RhoA antibody - ab86297 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RhoA antibody (ab86297) at 1 µg/mlLane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 : Human Platelet (Human adult normal cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-RhoA antibody - ab50540 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-RhoA antibody (ab50540) at 1/2000 dilutionLane 1 : Cell lysate of HEK-293 cells transfected with human RhoALane 2 : Cell lysate of HEK-293 cells transfected with human RhoA with 20 µg/mL immunizing peptide SecondaryHRP Goat Anti-Rabbit IgGdeveloped using the ECL technique

Anti-RhoA antibody - ab68829 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-RhoA antibody - ab75062 from Abcam

Host Sheep
Clonality Polyclonal
ab75062 (1/500) staining RhoA (red) and ab70221 staining Sortilin (green) in rat trigeminal tissue. DAPI counterstaining appears in blue.
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