ATP5B

Gene Symbol ATP5B
Entrez Gene 426673
Alt Symbol -
Species Chicken
Gene Type protein-coding
Description ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Other Description ATP synthase subunit beta, mitochondrial
Swissprots Q5ZLC5 P84168
Accessions Q5ZLC5 AJ719809 CAG31468 BU486121 CO506423 NM_001031391 NP_001026562
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Subcellular Location Mitochondrion {ECO:0000305}. Mitochondrion inner membrane. Note=Peripheral membrane protein.
Top Pathways Oxidative phosphorylation

Anti-ATPB antibody - ab150291 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab150291 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab150291 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ATPB antibody - ab177072 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ATPB antibody (ab177072) at 1/500 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal liver lysate

Anti-ATPB antibody - ab190295 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
All lanes : Anti-ATPB antibody (ab190295) at 0.3 µg/mlLane 1 : Human heart lysate (in RIPA buffer)Lane 2 : Mouse heart lysate (in RIPA buffer)Lane 3 : Rat heart lysate (in RIPA buffer)Lysates/proteins at 35 µg per lane.developed using the ECL technique
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