OTX2
| Gene Symbol | OTX2 |
|---|---|
| Entrez Gene | 395191 |
| Alt Symbol | - |
| Species | Chicken |
| Gene Type | protein-coding |
| Description | orthodenticle homeobox 2 |
| Other Description | homeobox protein OTX2|orthodenticle homolog 2 |
| Accessions | BAJ04682 AJ489221 CAD32962 AY600957 AAT44902 NM_204520 NP_989851 |
Anti-Otx2 antibody - ab154277 from Abcam
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Anti-Otx2 antibody - ChIP Grade - ab21990 from Abcam
![Otx1 +2 was immunoprecipitated using 0.5mg Y79 whole cell extract, 5µg of Rabbit polyclonal to Otx1 +2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Y79 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab21990.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 37kDa; Otx1 +2, non specific - as present in control (lane 2); 37kDa: We are confident this was due to slight lane contamination and the band seen in the IP lane is our target of interest.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5019_ab21990-199601-IPV024ab219901m30s.jpg)
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